储粮害虫米象磷化氢抗性分子机制研究

The rice weevil Sitophilus oryzae is one of the serious damage pest, which is resistance to phosphine (PH3). There are two hypothesis about the mechanism of PH3-resistanc, one is organophosphate insecticide resistant pattern, and the other is related to mitochondrial multienzyme complexes. The transcriptome of S. oryzae had been sequenced in our previous research. The resistant and susceptible strains will be selected and used for fumigating with PH3. Using transcriptome expression profiles technology, the candidate genes that regulate the PH3-resistanc will be isolated. The candidate genes about PH3-resistanc will also be selected from the genes related to the organophosphate insecticide and the mitochondrial multienzyme complexes by Real Time PCR method. Then, the key genes those regulate PH3-resistanc in S. oryzae will be further identified with RNAi technique. In the end, the possible molecular mechanism of PH3-resistanc will be obtained. The above results are useful for elucidating molecular toxicology mechanism of PH3 resistance in the rice weevil and other stored product pests, which can supply the theory basis for controlling the rice weevil.

米象是一种危害严重的粮食储藏害虫，目前已经对磷化氢（PH3）产生抗性。储藏害虫对PH3抗性目前存在经典的有机磷杀虫剂抗性机制和线粒体呼吸链抗性机制两种观点。在我们已经测定的米象转录组数据基础上，本项目首先建立米象PH3抗性和敏感品系，采取表达谱技术，比较抗性和敏感品系及PH3熏蒸后的基因表达水平；采用定量PCR技术，分析有机磷杀虫剂抗性机制和线粒体呼吸链抗性机制相关基因的表达模式，从三个角度筛选出与PH3抗性相关的候选关键基因。然后，进一步制备这些基因的dsRNA，采取RNAi技术鉴定这些基因的功能，明确调控米象PH3抗性的关键基因。最后，提出米象PH3抗性的可能的分子机制。研究结果将有助于阐明米象乃至其它储藏物害虫对PH3抗性产生和发展的分子毒理学机理，为控制米象的危害提供理论基础。