新型极性杯芳烃亲水作用色谱固定相的制备及保留机制研究

In the project, a series of novel stationary phases for hydrophilic interaction liquid chromatography (HILIC) will be developed to resolve the separation problems on polar components. A series of new HILIC bonded silica stationary phases with high affinity and adjustable separation mode will be designed and prepared using calixarenes as matrix and polar groups such as amino acid, sugar and ionic liquid as modifiers. The density and state of the "rich water layer" will be measured by a new technique of FM-type atomic force microscope (F-FAM), combined with density functional theory (DFT) and quantitative structure-retention relationship(QSRR), the interactions between HILIC stationary phase and solute molecules will be revealed qualitatively and quantitatively at the molecular level, and the new methods of separation and analysis of macrocyclic antibiotics and flavonoids, peptides and glycopeptides, nucleosides and nucleotides, heterocyclic amines and drug metabolites will be established. The project, which has important academic significance and practical value, will develop new HILIC stationary phases, clarify the HILIC mechanism and expand the application areas.

本项目针对强极性组分的分离难题，开展亲水作用液相色谱（HILIC）固定相研究。项目拟以硅胶为基质，以杯芳烃为母体，以氨基酸、糖、离子液体等极性基团为多元改性剂，设计和制备一系列具有高亲水性、分离模式可调控的新型HILIC固定相，利用调频型原子力显微镜（F-FAM）新技术表征测量“富水层”的密度和状态，结合密度泛函理论（DFT）和定量结构-色谱保留关系（QSRR），从分子水平上定性、定量地揭示HILIC固定相与溶质分子间相互作用的实质，建立大环抗生素与黄酮、多肽与糖肽、核苷与核苷酸、杂环胺与毒品代谢物等极性物质的分离分析新方法。本项目将开发新型HILIC固定相、阐明HILIC机制、拓展应用领域，具有重要的学术意义和实际应用价值。

本项目针对强极性组分的分离难题，选取硅胶为基质，以杯芳烃为母体，以氨基酸、糖、离子液体等极性基团为多元改性剂，设计和制备一系列具有高亲水性、分离模式可调控的亲水作用液相色谱（HILIC）固定相。同时，结合密度泛函理论（DFT）和定量结构色谱保留关系（QSRR），从分子水平上定性、定量地揭示了HILIC固定相与溶质分子间相互作用的实质，建立大环抗生素与黄酮、多肽与糖肽、核苷与核苷酸、杂环胺与毒品代谢物等极性物质的分离分析新方法。本项目对发新型HILIC固定相、阐明HILIC机制、拓展应用领域，具有重要的学术意义和实际应用价值。