绿盲蝽嗜好寄主茼蒿的高效引诱化合物分析

Resently years, Apolygus lucorum has become an important disaster factor on many industrial crops, such as cotton, grape, winter jujube, cherry, honey peach. We have found that Chrysanthemum coronarium with efficient attraction on Apolygus lucorum through preliminary study, but the active substance and constitution is undefined. Therefore, methods will be applied in this research program , such as SPME（solid phase microextraction），GC-EAD (gas chromatography-electroantennographic detection), GC-MS(Gas Chromatography-Mass Spectrometer）EAG（electroantennogram）. The plant volatile compound composition and content of information will be collected and identified , the chemical fingerprint library will be built, the green bugs behavioral responses on plant volatiles will be determined, he active component will be identified. On this basis, through the active component mixing, EAG response, determination of choice behavior, trap effect test in field, the compound compatibility will be determined. The results of this research program is significative not only for discussing the host selection behavior mechanism of plant hoppers and behavior regulation strategy but also for inaugurating a new sustainable control method on Apolygus lucorum,with wide prospect of application.

绿盲蝽Apolygus lucorum现已成为当前我国棉花、葡萄、冬枣、樱桃、水蜜桃等多种作物生产中的重要致灾因子，本项目前期研究发现茼蒿对绿盲蝽成虫具有很强的引诱活性，但其引诱活性物质及组分尚不明确。为此，本项目拟采用SPME、GC-EAD、GC-MS、EAG等技术收集和鉴定茼蒿植物挥发性信息化合物的组分与含量，构建化学指纹图谱库，研究绿盲蝽对植物挥发物的行为反应，分析鉴别对绿盲蝽具有吸引作用的活性组分，明确绿盲蝽与嗜好寄主之间的化学信息物质成分。在此基础上，通过活性组分混配、电生理反应与选择行为测定、田间诱集效果检验等筛选高效引诱绿盲蝽的化学配伍。研究结果不仅对探讨植食性昆虫的寄主选择行为机理与行为调控策略具有重要借鉴作用，且可开创绿盲蝽可持续防治新途径，应用前景广阔。

随着转Bt基因棉的大面积种植，绿盲蝽Apolygus lucorum （Meyer-Dur）由过去的次要害虫上升为棉田主要害虫，并在枣树、桃树、葡萄、梨树等多种农作物上暴发成灾。发展绿盲蝽无公害防治新技术非常迫切。通过本项目研究，明确了绿盲蝽对多种植物寄主的选择趋性，其中茼蒿对绿盲蝽的引诱性最强；利用GC-MS和GC-EAD分析了茼蒿的挥发性物质化学图谱，并筛鉴出绿盲蝽对其中6种化学物质具有较强的电生理反应；将分析得到的6种化学物质进行不同组合、不同剂量的混配，进行室内和室外的引诱活性检测，得到了3种具有利用价值的混配组合。本项目研究结果为开拓绿盲蝽新型生防技术奠定基础。