严重烧伤后脆弱类杆菌致病性的研究

We extracted LPS from cultured Fragilis with hydroxybenzene-water-petroleum method,analyzed SCFA in the medium by gas-liquid chromatograph .Then stimulated cultured human skin cells、PMN、PBMC、fibroblast、vascular endothelium cell with LPS or SCFA at different content, analyzed the change of cell's proliferation、apoptosis and function by the method of cytochemical、immunochemical、ELISA、Rt-PCR et al.we found SCFA can inhibit much function of PMN and fibroblasts,accelerate cell's apoptosis,reduce cell's proliferation;and LPS can increase the secretion of IL-2,decrease the secretion of IL-4.It showed that LPS and SCFA are important factor in the process of infection caused by Fragilis.

厌氧菌在烧伤感染中具有重要地位,本课题以烧伤厌氧菌感染的常见菌----脆弱类杆菌为研慷韵蠖韵?研究其外膜的致病性及其初步机理,对于阐明严重烧伤后厌氧菌感染的机理,有У胤乐紊丈烁腥揪哂兄匾砺酆土俅惨庖濉