基于抗氧化应激Nrf2/ARE/HO-1信号通路探讨艾灸干预阿司匹林诱导胃黏膜损伤的保护机制

As the NSAIDs use rate has multiplied, so that the risk of Nonsteroidal anti-inflammatory drug-induced gastropathy is greatly increased. The study found that the oxidative stress is one of the important parts in gastrointestinal injury,and Nrf2/ARE/HO-1 signaling pathway plays an important role in the effect of anti oxidative stress. TCM believes that the spleen qi and stomach qi deficiency syndrome is the crux of Nonsteroidal anti-inflammatory drug-induced gastropathy. Combined with previous studies, we put forward a new hypothesis:1.Moxibustion pretreated rats as subject, susing aspirin to intervene. Attempt to elaborate that moxibustion pretreatment can activate the Nrf2/ARE/HO-1 signaling pathway, increased anti oxidative stress ability, improving the gastric mucosal defensive repair capacity, thereby reducing the damage of gastric mucosal by aspirin. 2.Making aspirin induced gastric mucosal injury in rats as subject, using moxibustion therapy,compared with the HO-1 inhibitor, explore that moxibustion can activate the Nrf2/ARE/HO-1 signaling pathway, decreased oxidative stress reaction, repair of gastric mucosal injury induced by aspirin in rats.

随着非甾体抗炎药（NSAIDs）使用率成倍增长，使得非甾体相关性胃病的发病风险大大增加。研究发现：氧化应激是NSAIDs引起胃肠道损伤的独立因素之一，且Nrf2/ARE/HO-1通路在抗氧化应激中起着非常重要的作用。而中医认为脾胃气虚证为NSAIDs相关性胃病症结所在。结合前期研究，我们提出新的假说：1.艾灸预处理大鼠为受试对象，运用阿司匹林干预，试阐述艾灸预处理能激活Nrf2/ARE/HO-1信号通路，增加抗氧化应激能力，增强胃黏膜防御修复能力，从而减轻阿司匹林对胃黏膜的损伤；2.以阿司匹林诱导大鼠胃黏膜损伤为受试对象，采用艾灸治疗，以HO-1抑制剂为对照，探讨艾灸能激活Nrf2/ARE/HO-1信号通路，降低氧化应激反应，修复阿司匹林诱导的大鼠胃黏膜损伤，从而揭示艾灸防治阿司匹林防治阿司匹林诱导胃黏膜损伤的作用机制。

随着非甾体抗炎药（NSAIDs）使用率成倍增长，使得非甾体相关性胃病的发病风险大大增加。研究发现：氧化应激是NSAIDs引起胃肠道损伤的独立因素之一，且Nrf2/ARE/HO-1通路在抗氧化应激中起着非常重要的作用。而中医认为脾胃气虚证为NSAIDs相关性胃病症结所在。通过实验研究我们发现：1.艾灸预处理大鼠能增强胃黏膜保护能力，减轻阿司匹林对胃黏膜的损伤，艾灸大鼠能增强胃黏膜保护能力，修复阿司匹林对胃黏膜的损伤；2.艾灸预处理大鼠为受试对象，艾灸预处理能激活Nrf2/ARE/HO-1信号通路，增加抗氧化应激能力，增强胃黏膜防御修复能力，从而减轻阿司匹林对胃黏膜的损伤；3.以阿司匹林诱导大鼠胃黏膜损伤为受试对象，采用艾灸治疗，以HO-1抑制剂为对照，艾灸能激活Nrf2/ARE/HO-1信号通路，降低氧化应激反应，修复阿司匹林诱导的大鼠胃黏膜损伤。综上所述，艾灸预处理可增加胃黏膜抗氧化应激能力及艾灸能降低胃黏膜氧化应激反应，从而防治阿司匹林诱导胃黏膜损伤。