Ei24与糖尿病的关系及作用机制的研究

Cell apoptosis gene and autophagy gene, which involved in the development of diabetes, regulate pancreatic β cell number, proliferated ability and function, and play important roles in regulating concentration of blood glucose. Ei24, a kind of pro-apoptotic and autophagy related gene, could not only mediate the autophagy and apoptosis of normal cells, but also suppress the proliferation and infection progress of tumor cells. However, whether Ei24 take roles in the occurrence and development of type 2 diabetes is still unknown. We will use blood sample genome sequencing, fluorescence imaging, patch clamp recording, electron microscope to study the mechanism of Ei24 involved in regulating pancreatic β cell number, proliferated and secreted ability, and clarify the role of apoptosis and autophagy in the occurrence and development of type 2 diabetes.

细胞凋亡基因和细胞自噬基因调控着胰岛β细胞的数量、增殖和功能，参与了糖尿病的发生和发展过程，在血糖调控中发挥着重要的作用。Ei24是一种促凋亡且与细胞自噬相关的基因，参与了细胞自噬、凋亡发生、肿瘤细胞增殖和迁移等重要生理过程。但是其是否参与2型糖尿病的发生过程尚未见报道。前期我们尚未发表的数据表明，胰岛β细胞Ei24基因特异敲除小鼠表现出严重的葡萄糖耐受受损。本项目将利用血样基因组测序、荧光成像、膜片钳、电镜等多种生物物理、细胞生物学等手段，在分子、细胞以及动物层面研究Ei24调控胰岛β细胞数量、增殖和分泌的分子机制，阐明其是如何通过调节细胞凋亡或者细胞自噬来调节血糖代谢的作用机理，探讨细胞凋亡以及细胞自噬等过程在糖尿病发生和发展过程中的分子机制。