基于纳米金属有机框架(MOFs)荧光生物探针的病原微生物(Pathogen)高灵敏电化学快速检测方法研究

Contamination of Pathogen in water and food is an urgent national security issues, the high sensitive fast detection of Pathogen is fatal technology for the prevention and control of Pathogen contamination. However, the traditional detection methods still cannot satisfy the requirement of the high sensitive fast detection of Pathogen. In this work, to realize the high sensitive and accurately fast detection of Pathogen, the metal nano-particle functionalized graphene electrochemical substrate with high conductivity and good bioactivity is firstly prepared, then it is used for the immobilization of antibody and Aptamer which are specific for target pathogen to capture the target Pathogen in real sample onto electrode surface. On the other hand, the novel nano metal-organic frameworks (MOFs), a novel grid nano-material with permanent porosity and high surface area, is used as a supporter to prepare high fluorescence labeled and high enzyme labeled nano MOFs bio-probe through assembly high fluorescence carbon quantum dots (Cdots) on MOFs grid structure. Subsequently, the as-prepared novel MOFs bio-probes are innovatively used to fabricate the Aptamer based high sensitive electrochemical sensing platform for Pathogen fast detection. On the basis of the dual signal amplification strategy resulted from the excellent electrical conductivity of graphene electrochemical substrate and high fluorescence labeled MOFs bio-probe, the high sensitive fast detection of Pathogen in real sample is achieved without complex and time-consumed sample pretreatment process. Therefore, the present work definitely paves a way for the prevention and control of Pathogen contamination from both basic theory and creative technique.

病原微生物(Pathogen)污染是重大国家安全问题,其高灵敏快速检测是Pathogen污染防控关键环节。传统检测技术无法满足Pathogen快检需求。本研究以实现Pathogen(以大肠杆菌或沙门氏菌为模型)高灵敏快检为目标,制备高生物相容、高导电功能化石墨烯基底,将可特异性识别靶标Pathogen的抗体和适配体(Aptamer)固定其上以捕获靶标Pathogen。同时,以新型纳米金属有机框架(MOFs)为载体,利用其多孔结构和大比表面积,将荧光碳量子点标记其上制备多荧光标记MOFs生物探针,并创新性的将之用于构建基于Aptamer识别的Pathogen高灵敏电化学快检平台。基于高导电基底及MOFs生物探针对电化学信号的双重放大,无需繁杂样品前处理即可实现实际样品中靶标Pathogen的高灵敏快速检测,为Pathogen污染防控提供理论基础和创新技术。

本项目以纳米电化学和纳米运输载体为手段，开展相关的病原微生物检测和抗菌治疗方面的研究。在制备多种高导电及高比表面积的功能化石墨烯等纳米复合材料的基础上，对其组成和结构进行表征；研究适配体在所制备的纳米复合材料上的固定及对靶标分子的捕获技术，构建仿生纳米电化学界面；利用电化学阻抗检测技术研究靶标物质与纳米材料及适配体间的作用机制，发展新型的高灵敏高选择性的电化学传感器；研究光敏剂在功能化纳米材料上的负载机制和光动力抗菌作用机制，发展基于高光敏剂负载的功能纳米材料的光动力抗菌策略。为生物分析、临床诊疗等领域提供新材料、新技术和新方法。