酵母DNA体外复制的定点起始机制
基本信息
结项摘要
爪蟾卵抽提物可以使哺乳动物G1期细胞核的DNA复制在专一的位点上起始。另一方面，利用芽殖酵母S期的细胞抽提物，可以使芽殖酵母细胞核的DNA复制在专一的位点上起始。而在我们的研究工作中，我们发现爪蟾卵抽提物可以使G1期的芽殖酵母细胞核的DNA复制，但这种起始是非专一性的，不依赖特定的酵母DNA起始序列。这一工作揭示，芽殖酵母DNA复制的起始调控与哺乳动物细胞的不一样。这一工作还表明，爪蟾卵体外复制系统可以用来研究低等真核生物的DNA复制起始的调控。
项目摘要
项目成果
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数据更新时间：2023-05-31
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