清湿化瘀法对AM小鼠雌激素效应相关因子时空变化影响的研究

Adenomysis is a kind of disease which is very common among the women of reproductive age. It has complicated pathogenesis, causes severe symptom, and is very difficult to deal with . In traditional chinese medicine, it is generally accepted that stagnation of blood is the essential pathogenesis in development of adenomysis , so the therapy of dissipating blood stasis is the basal remedy. Combined with foundational theory and characteristic of our local area, we suggested that the interaction between dampness-heat and stagnation of blood is the most important pathogenesis of adenomysis in SiChuan province, and we have already gotten fairly good curative effect in clinical treatment by therapy of expelling dampness and dissipating blood stasis on local area, and we also have empirical study about it. On base of aboving work, we establish animal model of adenomysis by the method of transplating the pituitary gland of male mice into the uterus of the female mice, and then survey the content of estrogen and progesterone receptor on the level of mRNA and protein by the technique of Westernblot and RT-PCR respectively， detect the change of 17β-HSDI、17β-HSDⅡ、P450arom、COX2、PGE2 by the technique of immunohistochemisty. In the study, We evaluate the change of estrogen effect correlator before and after the treatment, study the distinction between the different groups and between ectopia and positional endometrium, in order to approach the mechanism of our existing theory.

子宫腺肌病(adenomyosis AM)是生育年龄妇女临床常见疑难疾病，针对AM发病机制复杂，临床危害大，难以治愈等特点，我们结合地域因素，提出"湿热瘀结"的病机学说，认为湿热与瘀血胶结难解、致病缠绵难愈。在临床应用清湿化瘀法治疗AM取得明显临床疗效和前期实验研究的基础上，运用同种异体垂体移植方法建立AM动物模型，采用 Westernblot和RT-PCR方法测定雌孕激素受体亚型在蛋白和mRNA水平的表达，免疫组化的技术检测17β-SDI、17β-SDⅡ、P450arom、COX2、PGE2变化。通过观察上述雌激素效应相关因子在AM发病及治疗前后随时间变化的表达差异，以及在各组别间的在位和异位内膜不同空间部位变化的表达差异，探究清湿化瘀法治疗AM的作用环节和作用靶点。以期探明清湿化瘀法通过调控雌激素效相关因子治疗AM的作用机制。

子宫腺肌病(adenomyosis AM)是生育年龄妇女临床常见疑难疾病，针对AM发病机制复杂，临床危害大，难以治愈等特点，我们结合地域因素，提出“湿热瘀结”的病机学说，认为湿热与瘀血胶结难解、致病缠绵难愈。在临床应用清湿化瘀法治疗AM取得明显临床疗效和前期实验研究的基础上，运用同种异体垂体移植方法建立AM动物模型，采用 Westernblot和RT—PCR方法测定雌孕激素受体亚型(ERα、ERβ、PR-A、PR-B、GPER）和Akt、p-Akt、NF-κB在蛋白和mRNA水平的表达，免疫组化的技术检测17β一HSD I、17β一HSD Ⅱ、P450arom、COX2、PGE2、ERα、ERβ、PR-A、PR-B、GPER、Ras、STAT3、Akt、p-Akt、NF-κB和PTEN的变化。本研究通过观察上述雌激素效应相关因子在AM治疗前后随时间变化的表达差异，及其在不同组别间的在位和异位内膜空间变化的表达差异，研究清湿化瘀法治疗AM的作用途径和作用环节。以期阐明清湿化瘀法通过调控雌激素效相关因子治疗AM的作用机制。