多价糖亲和温敏环形嵌段聚合物-盐双水相浮选体系的构建及其对植物凝集素的选择性分离纯化

The purification system of plant lectins is complex, and the obtained lectins has low-purity and serious activity loss, which becomes the bottleneck of the large-scale production of lectins. This topic is proposed to build a new method suitable for the separation and purification of lectins—multivalent glyco-affinity thermosensitive and cyclic block polymer–salt aqueous two-phase flotation, which can improve production efficiency, product purity, and at the same time reduce the activity loss of lectins. The main research contenst are as follows: ①Thermosensitive and cyclic block polymer is to be applied as phase-forming polymer of aqueous two-phase system, which can solve the problem of recycling difficulty in polymer and high viscosity of polymer-rich phase; ②The thermosensitive and cyclic block polymer–salt aqueous two-phase flotation system is to be established by coupling thermosensitive and cyclic block polymer–salt aqueous two-phase system and solvent sublation. The new system can realize process optimization on the whole, and solve the problem of relatively low enrichment factor of the single aqueous two-phase extraction method as well as environmental pollution and poor biocompatibility of the traditional solvent sublation method. ③ Amphipathic, glyco-affinity, thermosensitive and cyclic block polymer with multivalent effect is to be designed and synthesized to strengthen the selective distribution of lectins in the system, which can solve the poor selectivity and recycling problem of the traditional trapping agents. ④The related scientific problem is to be explored and solved, and it can guide the resolution of problems existing in application practice, making the technology can be used for large-scale separation and purification of biological products.

植物凝集素纯化体系复杂，所得凝集素纯度低，活性损失严重，成为了制约凝集素规模化生产的瓶颈。本课题拟构建一种适用于凝集素分离纯化的新方法—多价糖亲和温敏环形嵌段聚合物–盐双水相浮选，在提高生产效率和产品纯度的同时，有效降低凝集素的活性损失。主要研究内容如下：①将温敏环形嵌段聚合物用作双水相体系成相聚合物，解决目前成相聚合物难以分离回收以及聚合物相粘度较高的问题；②温敏环形嵌段聚合物-盐双水相体系与溶剂浮选技术耦合，构建温敏环形嵌段聚合物-盐双水相浮选体系，从整体上实现过程的优化，解决单一双水相萃取富集倍数不高以及传统溶剂浮选环境污染和生物相容性差的问题；③设计合成具有多价效应的两亲性含糖亲和温敏环形嵌段共聚物作为捕集剂来强化凝集素在体系中的选择性分配，解决传统浮选捕集剂选择性不佳以及分离回收的难题；④探索解决相关科学问题，指导解决应用实践中存在的问题，使该技术能用于生物制品的大规模分离纯化。

植物凝集素纯化体系复杂，所得凝集素纯度低，活性损失严重，成了制约凝集素规模化生产的瓶颈。本课题拟构建一种适用于凝集素分离纯化的新方法—糖亲和温敏嵌段聚合物–盐双水相浮选，在提高生产效率和产品纯度的同时，有效降低凝集素的活性损失。主要研究内容如下：①将温敏嵌段聚合物用作双水相体系成相聚合物，解决目前成相聚合物难以分离回收以及聚合物相粘度较高的问题；②温敏嵌段聚合物-盐双水相体系与溶剂浮选技术耦合，构建温敏嵌段聚合物-盐双水相浮选体系，从整体上实现过程的优化，解决单一双水相萃取富集倍数不高以及传统溶剂浮选环境污染和生物相容性的问题；③设计合成具有温敏聚合物-苯硼酸-糖嵌段聚合物作为捕集剂来强化凝集素在体系中的选择性分配，解决传统浮选捕集剂选择性不佳以及分离回收的难题；④探索解决相关科学问题，指导解决应用实践中存在的问题，使该技术能用于生物制品的大规模分离纯化。