基于金属螯合离子液体双水相气浮浮选体系的构建及选择性分离纯化植物蛋白酶的行为和机理研究

By combining the advantages of ionic liquid aqueous two-phase system (ILATPS), solvent sublation (SS) and metal chelate affinity, a metal chelate ionic liquid aqueous two-phase flotation system will be obtained and used to selective separation and purification of plant protease. An ILATPS which is used to separation and purification of plant protease is chosen by the construction of ILATPS, as well as the the thermodynamic and kinetic studies, and the property of the plant protease. Design and synthesize amphipathic pH or thermosensitive block copolymers with metal chelate moieties as trapping agents, and construct a metal chelate moieties based ionic liquid aqueous two-phase flotation system to improve separation selectivity for plant protease, implement the separation and purification of high-purity plant protease in complex systems. Meanwhile, implement recycling of ionic liquid and trapping agents based on pH or temperature response behavior of trapping agents. The related scientific problems of this topic will be deeply analyzed and resolved, and it will make this new method be applied to large-scale separation and purification of bioactive components in complex systems.

本课题旨在集离子液体-盐双水相体系、气浮溶剂浮选技术和金属螯合亲和作用的优点，构建金属螯合离子液体双水相气浮浮选体系，用于选择性分离纯化植物蛋白酶。通过离子液体-盐双水相体系的构建，以及热力学和动力学研究，并结合植物蛋白酶性质，筛选出适用于植物蛋白酶分离纯化的离子液体-盐双水相体系；设计合成金属螯合亲和配基的pH/温度响应型两亲性嵌段共聚物作为捕集剂，构建金属螯合离子液体双水相气浮浮选体系强化对植物蛋白酶分离的选择性，实现复杂基质中高纯植物蛋白酶的分离纯化；同时基于捕集剂的pH/温度响应行为，实现离子液体和捕集剂的回收和循环利用。深入分析并解决相关科学问题，提供一种复杂基质中大规模分离纯化生物活性组分的新方法。

本课题旨在集离子液体-盐双水相体系、气浮溶剂浮选技术和金属螯合亲和作用的优点，构建金属螯合离子液体双水相气浮浮选体系，用于选择性分离纯化菠萝蛋白酶。通过离子液体-盐双水相体系的构建，以及热力学和动力学研究，并结合菠萝蛋白酶性质，筛选出适用于菠萝蛋白酶分离纯化的离子液体-盐双水相体系；设计合成金属螯合亲和配基的温度响应型两亲性嵌段共聚物作为捕集剂，构建金属螯合离子液体双水相气浮浮选体系强化对菠萝蛋白酶分离的选择性，实现复杂基质中高纯植物蛋白酶的分离纯化；同时基于捕集剂的温度响应行为，实现离子液体和捕集剂的回收和循环利用。深入分析并解决相关科学问题，提供一种复杂基质中大规模分离纯化生物活性组分的新方法。