白杨素调控自噬影响脂肪细胞增殖分化及脂质代谢的作用与机制研究

Recently, obesity and related complicating diseases caused by disorders of lipid metabolism have become a worldwide hygiene problem. Chrysin is one kind of flavonoid compounds that can be extracted from many animals and plants. It has been reported that chrysin has the capacity of regulating cell autophagy, while the latter plays important roles in proliferation and differentiation of the preadipocyte, as well as in accumulation and degradation of lipid. The potential function of chrysin in regulating lipid metabolism and its mechanism require further investigation. The proposed study aims to explore the role of autophagy in chrysin regulating lipid metabolism through in vivo and vitro experiments. The effects of chrysin on lipid metabolism in mice with dietary-induced obesity and differentiation of preadipocyte, and the roles of autophagy will be investigated; then the key genes related to autophagy will be silenced by siRNA technique, to check whether autophagy is the pathway of chrysin regulating lipid metabolism; also through introducing of autophagy inducer, the possibility of increasing the susceptibility of preadipocyte to chrysin will be investigated. In conclusion, the proposed research aims to illuminate the effect and mechanism of chrysin regulating lipid metabolism, also this research will provide experiment evidences and theoretical basis for exploring application of chrysin in disorders of lipid metabolism and related obesity.

近年来，脂质代谢异常引起的肥胖及各种并发症已成为全球卫生领域的重要课题。从天然动植物成分中筛选能调控脂质代谢的化合物，是国内外研究的重要方向之一。黄酮类化合物白杨素，被报道可以调控细胞自噬。而自噬在前脂肪细胞的分化及脂肪细胞内脂滴的沉积与降解中有重要作用。白杨素潜在的调控脂质代谢的作用及其机理有进一步研究的必要。本项目将结合体内及体外实验，研究白杨素对食源性肥胖小鼠脂质代谢及前脂肪细胞分化的影响，以及自噬在此过程中发挥的作用。进一步利用siRNA技术沉默自噬关键基因，验证自噬是否是白杨素调控脂质代谢的关键通路；同时探索自噬诱导剂能否增强脂肪细胞对白杨素的敏感性。通过以上研究，本项目将阐明白杨素调控脂质代谢的作用及其机制，并能够为白杨素提取物应用于脂质代谢异常及肥胖的防治提供实验依据和理论基础。