水稻盐胁迫下特异表达基因的筛选及克隆研究

The areas suffered from salt damage could account for 1/5 of rice cultivated areas in China and have an increasingly enlarging and deteriorating trend. Salt damage is becoming a restricted factor of agricultural development. In this study, 3 positive clones were gained by using differential screening method and mapped on the genetic map derived from a DH population. Simultaneously, compared these clones with the QTLs for controlling salt-tolerance, determined that the positive clone is a candidate gene for QTL of salt-tolerance, and Primarily analyzed the mechanism of rice differential expressing gene under salt stress. In addition, conducted still the research for gene engineering of rice salt tolerance by transforming a few genes to the same plant, and got some transgenic plants with a 1 % NaCI tolerance. This finding is awarded Zhejiang Province Sci-Tech achievement identification and Sci-Tech award 2 for the Chinese Academy of Agricultural Science.

盐害是制约农业发展的一个严重问题，我国水稻可耕面积中，大约1/5受到盐害侵染，对水狙魏浦跎佟１臼笛槟獠捎靡呀ǔ傻聂痰咎厝?号的盐处理及对照两个cDNA文库，利用差示筛选方法，分离水稻盐胁迫下特异表达基因，将其定位到DH群体构建的染色体图谱，比较cDNA与耐盐QTLs之间遗传距离，为耐盐QTLs克隆及分子育种提供依据。