基于DNA组装的耦合增强SERS纳米探针用于mRNA检测及活细胞成像

Surface enhanced raman scattering holds great promise for detection and biological imaging in virtue of its high sensitivity, fast detection, narrow peak, no destruction, not subject to spontaneous fluorescence and water interference. However, it has been a great challenge to construct sensitive SERS nanoprobes which are structureally controllable and reproducible. Based on DNA hybridization mediated assembly of nanoparticles，the project focuses on the development of novel dimer and pyramid coupling enhanced SERS nanoprobes which is highly controllable, and research on SERS enhancement mechanism. As well, a novel technique will be developed on coupled enhanced SERS nano-probe for live cell biosensor, which cancer-related messenger RNA as a model. Furthermore, combining with complex SERS probe construction technology, multiple target synchronization detection will be explored. A new platform will be constructed for real-time SERS imaging analysis of intracellular cancer markers with high throughput and high sensitivity.

表面增强拉曼散射（Surface Enhanced Raman Scattering, SERS）技术以其灵敏度高、检测速度快、谱峰窄、无损且不受自发荧光和水的干扰等优点，在分析检测和生物成像中展现出显著的优势。然而构建结构可控的、重现性好的高灵敏的SERS纳米探针仍具有挑战性。本项目拟基于DNA杂交介导的纳米组装技术，设计合成结构高度可控的二聚体、金字塔等离子体纳米探针，开展SERS增强机理研究，并以癌症高度相关的mRNA为模型，发展耦合增强SERS纳米探针的活细胞生物传感新技术；在此基础上，结合多色SERS探针构建技术，发展多种mRNA同步高灵敏检测的耦合增强 SERS生物传感技术，构建活细胞内癌症标记物高通量、高灵敏度的实时SERS成像分析新方法，为相关的生物学研究和疾病早期预防及诊断提供新的技术平台。

准确获取生物体内重要生化信息对于实现疾病早期诊断和治疗有着重大意义，基于DNA组装的信号放大技术、纳米生物探针技术已成为准确获取生化信息的强有力研究工具。本项目采用等温核酸放大技术、纳米组装技术系统构建几种癌症标志物检测平台，并将它们成功应用于细胞/活体中生物活性分子的高灵敏度、高选择性荧光检测荧光成像。本项目所取得的成果将为智能响应性荧光探针材料的设计合成与生物成像应用研究等方面提供了新思路。从项目立项至今，从项目立项至今， 已先后在ACS SENSORS（1 篇）、Sensors and Actuators B: Chemical（2 篇）、 Chinese Chemical Letters（1 篇）等国际国内学术期刊上发表相关工作 9 篇学术论文，其中影响因子大于 5.0 的论文 6 篇，申请国家发明专利1篇。顺利完成了预期成果。