莲草直胸跳甲对CO2 浓度升高的种群生理生殖响应机制

In our preliminary studies, the phenomenon of elevated CO2 effect on the population growth of flea beetle, Agasicles hygrophila was found. In.view of this phenomenon, we will use the theories and techniques of population life table, physiology and chemistry, behavior analysis.to reveal the population adaptation strategy of flea beetle, A. hygrophila to elevated CO2 concentration. The key research contents include: (1)The effect of elevated CO2 on the survival rate, development of flea beetle, which clarify the multiplication strategy of A. hygrophila will be measured; (2)The effect of elevated CO2 on the protein, sugar content, respiration metabolism and enmyze activities of the third larvae of A. hygrophila will be analyzed, which explicit the metabolism and protect machanism of A. hygrophila under elevated CO2; (3) distinguish the difference of reproductive organs, behavior and endocine regulation changeof flea beetle under elevated CO2, which clear the physiology response machanism of A. hygrophila under elevated CO2. The implementation of this project will explore the adaptability and response machanism of A. hygrophila in response to elevated CO2 through three aspect of physiology, individual and population. The results were help to enrich the evolution theory of insect adaptability, which can provide the theoretical and practical basis on the biological control of Alternanthera iloxeroides in the future global warming.

针对大气CO2 浓度升高对莲草直胸跳甲种群增长产生影响的现象，围绕莲草直胸跳甲对大气CO2 浓度升高的种群增殖适应策略这一科学问题，采用种群生命表、生理生化测定、个体行为分析等研究手段，重点研究：（1）不同CO2 浓度胁迫莲草直胸跳甲种群的存活、发育和种群生命表参数，明确莲草直胸跳甲响应大气CO2 浓度升高的生存与种群增殖策略变化；（2）不同浓度CO2 胁迫种群三龄幼虫的体内蛋白、糖含量、呼吸代谢酶活性差异，明确莲草直胸跳甲在不同CO2 浓度条件下的生理代谢特性与保护机制；（3）不同CO2 浓度胁迫种群的生殖器官、行为和分内泌调控变化，揭示莲草直胸跳甲对CO2 浓度升高的生殖响应机制。预期结果从生理、个体代谢和种群三个不同的层面阐明莲草直胸跳甲对大气CO2浓度升高的适应性与响应机制，结果有助于丰富昆虫适应性进化理论，为指导莲草直胸跳甲的大量繁殖和空心莲子草的生物防治提供理论和实践依据

针对大气CO2浓度升高对莲草直胸跳甲种群增长产生影响的现象，围绕莲草直胸跳甲对大气CO2浓度升高的种群增殖适应策略这一科学问题，采用种群两性生命表、生理生化测定、个体行为分析等研究手段，重点研究了：（1）不同CO2浓度胁迫莲草直胸跳甲种群的存活、发育和种群生命表参数，明确莲草直胸跳甲响应大气CO2浓度升高的生存与种群增殖策略变化；（2）不同浓度CO2胁迫种群三龄幼虫的体内蛋白、糖含量、呼吸代谢酶活性差异，明确莲草直胸跳甲在不同CO2浓度条件下的生理代谢特性与保护机制；（3）不同CO2浓度胁迫种群的生殖器官、行为和分内泌调控变化，揭示莲草直胸跳甲对CO2浓度升高的生殖响应机制。.研究结果表明，（1）在高浓度CO2条件下，莲草直胸跳甲卵、1龄幼虫和蛹的发育历期缩短，而3龄幼虫和幼虫总发育历期显著延长；内禀增长率、周限增长率和净增值率增加，世代历期显著缩短。（2）高CO2浓度处理下，莲草直胸跳甲幼虫取食量显著增加；3龄幼虫体内蛋白含量显著增加，总氨基酸含量差异不显著；莲草直胸跳甲3龄幼虫体内消化酶（淀粉酶和胃蛋白酶）的酶活力显著降低。（3）莲草直胸跳甲成虫分别在雌雌和雄雄条件下的相遇行为主要表现为避让、打斗2种方式，且相互避让的频率明显高于打斗的频率；雌雄成虫相遇行为主要以避让为主，雌雄成虫多次相遇后可能发生交配，但交配的频率相对较少。莲草直胸跳甲雌性生殖系统包括卵巢、侧输卵管、中输卵管、受精囊及其附腺等，雄性生殖系统由睾丸、输精管及附腺、射精管及附属器官等组成。.研究结果从生理、个体代谢和种群三个层面阐明了莲草直胸跳甲对大气CO2浓度升高的适应性与响应机制，有助于丰富昆虫适应性进化理论，为揭示未来全球气候变化下莲草直胸跳甲的生存适应性及其对空心莲子草的防控能力评估提供科学依据，为指导莲草直胸跳甲的大量繁殖和空心莲子草的生物防治提供理论和实践依据。