高CO2浓度促进莲草直胸跳甲种群繁殖的表观跨代效应机制

The global climate change caused by the increase of CO2 concentration is one of the most important environmental problems. The rapid change of insects' survival and reproduction ability is an important strategy to adapt to the environment. Response mechanism of insects to environmental changes may be related to epigenetics transgenerational inheritance. Previous studies showed that elevated CO2 promoted the population reproduction of Agasicles hygrophila. Therefore, we speculate that there is epigenetics transgenerational inheritance in the adaptability of A. hygrophila to elevated CO2. The present program focuses on the key scientific issue of the adaptability of natural enemies to elevated CO2 concentration, and takes the natural enemy of the important invasive alien weed, Alternanthera philoxeroides, as the research object. Three experiments will be conducted. First, maternal and/or paternal effects in the transgenerational inheritance of A. hygrophila under elevated CO2 will be studied. Subsequently, methylation-dependent restriction-site associated DNA sequencing (MethyRAD-Seq) technology will be used to detect and analyze the levels and patterns of DNA methylation of A. hygrophila in different generations. Finally, cloning and functional verification of the full-length cDNA sequence of Dnmt1 gene of A. hygrophila in different generations will be conducted to explore the regulatory role of DNA methylation in its adaptation to the transgenerational effect under elevated CO2. Our results will provide important scientific significance and reference for the biological control of A. hygrophila against A. philoxeroides in elevated CO2 concentration, and may also help assess the impacts of climate change on insects.

大气CO2浓度升高引起的全球气候变化是最主要的环境问题之一。昆虫存活和繁殖能力的快速变化是其适应恶劣环境生存的重要策略，昆虫对环境变化产生的响应机制可能与表观遗传有关。前期研究表明，高CO2浓度促进莲草直胸跳甲种群繁殖，推测莲草直胸跳甲对高CO2浓度产生了适应性的表观跨代遗传。本项目关注天敌昆虫对大气CO2浓度升高的适应性这一关键科学问题，以入侵杂草空心莲子草的天敌莲草直胸跳甲为研究对象，重点研究：CO2浓度升高下，莲草直胸跳甲跨代遗传中的母性和父性效应；采用MethyRAD-Seq技术分析不同世代莲草直胸跳甲的DNA甲基化水平及模式；克隆不同世代莲草直胸跳甲的Dnmt1基因的全长cDNA序列并进行功能验证，探索甲基化在跳甲适应CO2浓度升高跨代效应中的调控作用。研究结果为莲草直胸跳甲在大气CO2浓度升高环境下对空心莲子草的生物防治和研究气候变化对昆虫的影响具有重要的科学意义和参考价值。

大气CO2浓度升高引起的全球气候变化是最主要的环境问题之一。昆虫存活和繁殖能力的快速变化是其适应恶劣环境生存的重要策略，昆虫对环境变化产生的响应机制可能与表观遗传有关。前期研究表明，高CO2浓度促进莲草直胸跳甲种群繁殖，推测莲草直胸跳甲对高CO2浓度产生了适应性的表观跨代遗传。本项目关注天敌昆虫对大气CO2浓度升高的适应性这一关键科学问题，以入侵杂草空心莲子草的天敌莲草直胸跳甲为研究对象，重点研究：CO2浓度升高下，莲草直胸跳甲跨代遗传中的母性和父性效应；采用全基因组甲基化测序（Whole Genome Bisulfite Sequencing, WGBS）分析不同世代莲草直胸跳甲的DNA甲基化水平及模式；克隆不同世代莲草直胸跳甲的Dnmt1基因的全长cDNA序列并进行功能验证，探索甲基化在跳甲适应CO2浓度升高跨代效应中的调控作用。研究结果为莲草直胸跳甲在大气CO2浓度升高环境下对空心莲子草的生物防治和研究气候变化对昆虫的影响具有重要的科学意义和参考价值。