间充质干细胞的超弱光子辐射特性研究

Owing to the unique potential of self-renewal and multi-lineage differentiation, stem cells will find usefulness in repairing and regenerating diseased or aging tissues and organs, restoring impaired body functions. Therefore, stem cells have great medical value and broad application prospects. As the research and clinical applications of stem cells become increasingly widespread, to explore a label-free and non-destructive technology for stem cells' detection has become an urgent need, which is significant for the industrialization of stem cells. Bio-photon technology has the potential to become a promising option. It is able to acquire biological information by detecting and analyzing the ultra-weak photon emission from the bio-systems, with high sensitivity and specificity. Related works regarding using this technology for stem cells' detection have not yet been reported abroad. In our previous work, the photo-induced delayed luminescence of mesenchymal stem cells was found to show regular changes with the cell viability decreased, and the potential of this technology in stem cells' detection was confirmed. Based on these findings, we will further study the mesenchymal stem cells' ultra-weak photon emission, including spontaneous photon emission and photo-induced delayed luminescence, as well as their relations with the cellular physiological states such as cell cycle, viability and aging. This work will lay the foundation for using bio-photon technology to develop a non-destructive means for stem cells' detection, and also provide the basis for in-depth exploration of bio-photon emission mechanisms.

干细胞以其独特的自我更新与多向分化潜能，有望从根本上修复和再生病变或衰老的组织器官，恢复受损的机体功能，极具医学价值和应用前景。随着干细胞研究和临床应用的日益广泛，探索无外源性标记、无损的干细胞检测技术已成为干细胞产业化发展中急需研究的重要课题。生物光子技术有望成为一个新的选择，它通过探测和分析生物系统的超弱光子辐射特性，获得被测样品的生物信息，具有高度的灵敏性和特异性。将生物光子技术用于干细胞研究，迄今国外尚无报道。在前期研究中，我们发现间充质干细胞的光诱导延迟发光动力学参数随细胞活力下降呈现规律性变化，证实了该技术在干细胞检测中的应用潜力。为进一步深入研究，我们将从自发光子辐射和光诱导延迟发光两方面，系统研究间充质干细胞的超弱光子辐射特性及其与细胞周期、活力、衰老等生理状态变化的关联，为利用生物光子技术发展一种干细胞无损检测手段奠定基础，同时也为深入探索生物光子辐射机理提供依据。

干细胞以其独特的自我更新与多向分化潜能，有望从根本上修复和再生病变或衰老的组织器官，恢复受损的机体功能，极具医学价值和应用前景。随着干细胞研究和临床应用的日益广泛，探索无外源性标记、无损的干细胞检测技术已成为干细胞产业化发展中急需研究的重要课题。本项目采用生物超弱光子检测技术、显微拉曼光谱技术和自体荧光光谱技术，研究了间充质干细胞本征的光学特性及其与细胞生理状态的关联，取得的主要研究结果如下：1、改进了活体细胞生物光子检测系统，首次测得了持续长时间（超过1秒）的细胞光诱导延迟发光信号；2、系统研究了间充质干细胞的光诱导延迟发光特性，论证了其衰减动力学模型为双曲衰减模型，揭示了光照参数、细胞密度、细胞数量等因素对细胞延迟发光特性的影响，首次发现细胞密度增大及细胞数量增多会导致细胞延迟发光衰减变慢、寿命延长；3、基于这些实验结果探讨了细胞延迟发光的来源和物理机制，提出细胞延迟发光来源于细胞之间的光子传递效应，为证明生物光子辐射的相干理论提供了新的实验依据；最后，研究了细胞活力、衰老等生理状态变化与细胞延迟发光、拉曼光谱、自体荧光光谱等光学特性的关联，揭示了对间充质干细胞生理状态变化敏感的光学指标。这些结果为利用光学技术发展干细胞无损检测手段奠定了基础，同时也为深入探索生物光子辐射机制提供了依据。