离子液体作为流动相添加剂应用在高效液相色谱中的色谱行为规律及机理研究

The different basic components could be studied on the fact that the separation effective and good stability were obtained by using mobile phase containing room temperature ionic liquids as additive. The chromatographic behavior could be also studied by using thermodynamic parameter and chromatographic dynamics theory to clarify the mechanism for the separation of components with a mobile phase containing room temperature ionic liquids as additive. The comprehensive thorough research on separation method and mechanism could be studied and confirmed by investigating the factors influencing the chromatographic behavior and establishment of separation model, which provides reference for application of ionic liquid on HPLC.

本课题针对“离子液体加入流动相作添加剂，能够增强碱性或极性组分分离效果和分离稳定性”这一实验现象，选取不同碱度组分为研究对象，借助色谱热力学模型和动力学因素考察的研究方法，通过系统探讨离子液体作流动相添加剂的色谱行为规律，结合热力学参数和动力学因素的相关性，以阐明离子液体在色谱分离过程中的作用机理；进而通过动力学因素变化规律，明确影响柱效的关键因素，并最终对离子液体作流动相添加剂的高效液相色谱分离法进行全面深入研究，为离子液体的在液相色谱分离中的应用研究提供借鉴。

本课题选取不同碱度生物碱组分为研究对象，结合色谱学参数及相关热力学模型，系统探讨离子液体作为流动相添加剂的色谱行为规律。研究中首次发现：（1）在以离子液体为流动相添加剂分离生物碱的色谱方法中，组分的色谱行为随生物碱碱度的不同而不同。（2）在研究各组分色谱行为规律时，引入溶质计量置换保留模型（SDM-R）和Snyder模型，阐明了离子液体在色谱分离过程中的规律及作用机理。（3）考察动力学因素变化，明确相关因素的影响。系统全面的建立了以离子液体为流动相添加剂分离生物碱的色谱方法，并阐明分离机理。