光调节源库关系及影响胡椒花量的机理研究

Black pepper needs coordinated source-sink relation to obtain high yield. Light intensity is the key factor that influence the photoassimilate accumulation and partitioning, however, the mechanism of light intensity regulate source-sink relationship, and then affect the pepper inflorescence quantity is not yet clear. Therefore, it is critical to reveal the mechanism for improving pepper yield and quality. In the study, shade net technology under field conditions in combination with indoor light intensity simulation experiment is adopted, by measuring the organs, which indicate source-sink strength, key sucrase activity of carbon metabolism, and source-sink volume. Quantificationally analyze the effect of light intensity to source-sink strength as well as use 13C isotopically tracer method explore the regulation metablism of light intensity to black pepper photoassimilate accumulation and partitioning.Then build source-sink strength parameter system of black pepper under light intensities in different development stage, and clear the effect of source-sink strength to black pepper leaf photoassimilate accumulation and partitioning. Furthermore, propose critical light intensity of high yield and quality black pepper production.Finally, explore the mechanism of light intensity regulates source-sink relationship and control inflorescence. These not only help determine black pepper optimum source-sink strength in different growth development period, but also make further understanding of the photoassimilate partitioning mechanism of black pepper different growth development stage and then provide the scientific basis for optimizing inflorescence regulation of black pepper production and cultivation pattern.

胡椒需要源库协调发展才可以获得高产，光强是影响光合同化产物累积和分配的关键因素，然而光强调控源库关系进而影响胡椒花量的机理尚不清楚，解析这一机理是实施胡椒高产优质生产的关键。本研究拟采用遮阳网田间原位遮荫技术，结合室内光强模拟试验，通过动态测定胡椒指示源库强度的器官中关键蔗糖酶活性和源库容量，定量分析不同光强对胡椒源库强度的影响；利用13C示踪法，探明光强调控光合同化产物累积和分配的机理，建立不同光强条件下胡椒不同生长阶段的源库强度指标体系，明确源库强度对胡椒叶片光合同化产物累积和分配的影响，提出高产优质胡椒生产的适宜光强，揭示光强调控源库调节花量的机理。研究成果不仅有助于确定胡椒不同生长发育时期适宜源库强度，而且为深入剖析胡椒不同生育阶段的光合产物分配机理奠定基础，并为优化胡椒生产中花期调控和栽培模式提供科学依据。

胡椒源库协调发展才可以获得高产，光强是影响光合同化产物累积和分配的关键因素，然而光强调控源库关系进而影响胡椒花量的机理尚不清楚，解析这一机理是实施胡椒高产优质生产的关键。本研究采用遮阳网田间原位遮荫技术，结合室内光强模拟试验，通过动态测定胡椒指示源库强度的器官关键糖代谢过程和激素水平相关指标，分析了不同光强条件下碳代谢过程和激素水平对胡椒源库强度的影响，发现了低光强条件下，源强对胡椒花量的负调控作用；利用13C示踪法，探明了光强调控光合同化产物在源库的累积和分配能力影响了源库强度，进而影响花量的机理，提出高产优质胡椒的生产需要适宜光强调控源端碳分配和赤霉素水平来实现。研究成果不仅有助于确定胡椒源强调控参数，而且为深入剖析胡椒不同生育阶段的光合产物与激素互作的调花机理奠定了基础，并为优化胡椒生产中调花技术提供了科学依据。