血小板活化因子及其受体在创伤性脑损伤中的作用研究

The Incidence,deformity rate and lethality induced by traumatic brain injury(TBI) is very high,and it threatens the life of human beings. The neuroinflammation in central never system after TBI plays an important role in the secondly brain injury. platelet-activating factor(PAF) is the most strong pro-infalmmation cytokine.Our research group had studied the role of PAF in the central nerve system disease for a long time. We found that PAF receptor antagonist could obviously improve the recovery of brain injury. It suggested that PAF receptor antagonist can be the new target of TBI treatment. The contents of this research are in follow aspects: how do PAF and its receptor effect the pathophysiology of neurons cultrued in vitro? How do PAF and its receptor effect formation of long-term potentiation(LTP) of the brain section cultured in hypoxia condition? RNA interference technology was used to silent the PAF receptor gene of fetal rats, rats were made TBI model by hydraulic attacking, then observing whether PAF and its receptor can improve the cognition and behavior of model rats or not? Whether can prevent neuroinflammation and neurons death or not? Solotion of these problems could provide theory basis for TBI pathophysiology and clinical treatment.

创伤性脑损伤（traumatic brain injury, TBI）发病率、致残率和致死率极高，严重威胁着人类的生命。TBI后中枢神经系统的炎症反应是继发性脑损伤的重要原因之一。血小板活化因子（platelet-activating factor，PAF）是迄今发现的作用最强致炎因子。课题组对PAF及其受体在中枢神经系统疾病中的地位及作用进行了长期研究，发现PAF受体拮抗剂能明显促进脑创伤后的恢复。提示PAF受体拮抗剂为TBI新的治疗靶点。本课题研究PAF及其受体对缺氧培养神经细胞的细胞病理生理学影响；能否促进缺氧状态海马脑片长时程增强(LTP)的形成？采用RNA干扰技术沉默大鼠PAF受体基因，使用液压打击TBI模型，PAF受体拮抗剂对动物认知和行为是否有所改善？能否防止脑内炎症反应及神经死亡？这些问题的解决将为TBI的病理生理及临床治疗提供理论体依据。

创伤性脑损伤（traumatic brain injury, TBI）发病率、致残率和致死率极高，严重威胁着人类的生命。TBI后中枢神经系统的炎症反应是继发性脑损伤的重要原因之一。血小板活化因子（platelet-activating factor，PAF）是迄今发现的作用最强致炎因子。课题组对PAF及其受体在中枢神经系统疾病中的地位及作用进行了长期研究，发现PAF受体拮抗剂能明显促进脑创伤后的恢复。提示PAF受体拮抗剂为TBI新的治疗靶点。本课题研究PAF及其受体对缺氧培养神经细胞的细胞病理生理学影响；能否促进缺氧状态海马脑片长时程增强(LTP)的形成？采用RNA干扰技术沉默大鼠PAF受体基因，使用液压打击TBI模型，PAF受体拮抗剂对动物认知和行为是否有所改善？能否防止脑内炎症反应及神经死亡？这些问题的解决将为TBI的病理生理及临床治疗提供理论体依据。