脂肪组织特异表达线虫脂肪酸脱氢酶小鼠模型的建立
基本信息
结项摘要
多不饱和脂肪酸（PUFA）在人类和哺乳动物体内不能自身合成，必须从食物中获取。其中n-3和n-6族PUFA具有重要功能，尤其是n-3族PUFA可促进发育和预防多种疾病。而线虫fat-2基因编码的Δ12脂肪酸脱氢酶可将单不饱和脂肪酸转化为n-6族PUFA，fat-1基因编码的n-3脂肪酸脱氢酶进一步将n-6族PUFA转化为n-3族PUFA。本项利用aP2启动子构建fat-1基因的脂肪组织特异表达载体，借助原核注射获得表达n-3脂肪酸脱氢酶的转基因小鼠。在此基础上，将fat-2与fat-1基因偶联，构建同时特异表达两个基因的载体，实现小鼠体内n-6、n-3族PUFA的自身合成。分析转基因小鼠各组织PUFA含量及生理特征可能发生的改变。以此探讨基因导入对脂肪代谢的影响，为利用转基因技术改良家畜脂肪成分提供模型。并对n-3和Δ12脂肪酸脱氢酶进行细胞器定位，为研究PUFA代谢机制提供参考。
项目摘要
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数据更新时间：2023-05-31
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