高通量水产品脂质组学方法研究与数据库初建

China is the world's largest aquatic products producer and trader. However, lipidomics research of aquatic products is still lagged behind when compared with that in other countries, due to the lack of an efficient method. In this project, TiO2 microwell array chip (TMAC) is synthesized by photocatalytic reaction, and the conditions, including pressure, spraying time, and deposition layers, are optimized to make the synthetic steps simple and the size of chip controllable. By combining with the matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (MALDI-TOF/MS), a high-throughput lipidomics method is established, which makes the time for phospholipids enrichment and detection less than 5 minutes and 5 seconds, respectively. Afterwards, the linearity, limits of detection and quantification, precision, and recovery of the TMAC-MALDI-TOF/MS method are validated. The method is applied to major aquatic species to get the lipidomics big data. The relationship and specificity of phospholipids in aquatic product with different species, origin, living environment are investigated and discussed. The application of this technique not only be very meaningful for efficiently comprehensive utilization of aquatic production, but also provide scientific reference and evidence for species identification, source tracing, diagnosis, pollution monitoring, and so on.

中国是世界最大的水产品生产和贸易国，但水产品脂质组学研究相对国际还十分落后，原因之一是缺乏高效的脂质组学研究方法。本项目利用TiO2对磷脂的选择吸附特性，通过光催化反应合成TiO2微孔阵列芯片；研究压力、喷雾时间、淀积层数等参数对芯片物理与化学性质的影响，使合成操作简易，芯片尺寸可控。联用基质辅助激光解吸飞行时间质谱建立高通量脂质组学方法，将多水产样品磷脂的富集时间控制在5分钟，单样品检测时间控制在5秒钟。对该方法的线性、检出和定量限、精密度及回收率等方面进行方法学验证，并对中国主要经济水产品进行脂质组学研究，快速获取水产品脂质组学大数据，深入探讨不同种属、地域及环境胁迫下水产品脂质特异性与相关性，初步建立水产品脂质组学数据库。该技术在水产品研究中的应用，不仅对水产品高效综合利用有重要意义，还为水产品鉴伪、溯源，诊断水生动物疾病提供参考，也为水环境调控与污染监测提供科学依据。

中国是世界最大的水产品生产和贸易国，但水产品脂质组学研究相对国际还十分落后，原因之一是缺乏高效的脂质组学研究方法。本项目利用TiO2对磷脂的选择吸附特性，通过光催化反应合成TiO2微孔阵列芯片；研究压力、喷雾时间、淀积层数等参数对芯片物理与化学性质的影响，使合成操作简易，芯片尺寸可控。联用基质辅助激光解吸飞行时间质谱建立高通量脂质组学方法，将多水产样品磷脂的富集时间控制在5分钟，单样品检测时间控制在5秒钟。对该方法的线性、检出和定量限、精密度及回收率等方面进行方法学验证，并对中国主要经济水产品进行脂质组学研究，快速获取水产品脂质组学大数据，深入探讨不同水产品脂质特异性与相关性，初步建立水产品脂质组学数据库。该技术在水产品研究中的应用，不仅对水产品高效综合利用有重要意义，还为水产品鉴伪、溯源，诊断水生动物疾病提供参考，也为水环境调控与污染监测提供科学依据。