致病相关色素合成蛋白Vayg1调控大丽轮枝菌侵染马铃薯的作用机制研究

Verticillium wilt of potato caused by Verticillium dahliae, also called potato early dying disease, is an important factor limiting potato production in the world. Currently, the poor understanding of the pathogenic mechanism of Verticillium dahliae has led to increasingly severe challenges in the effective control of Verticillium wilt. Many studies have shown that pigment biosynthesis related proteins are virulence factors of Verticillium dahliae, while the regulatory mechanisms are still unknown. In order to elucidate the pathogenic mechanism regulated by the virulence-related pigment biosynthesis protein Vayg1 in Verticillium dahliae, proteomic analysis will be used to judge the Vayg1 deletion mutant and the wild-type strain during the infection of potato to explore the Vayg1-involved pathogenic protein network, aiming to identify and understand relevant pathogenic pathways to provide an important support for deeper understanding of the pathogenic mechanism of the pathogen. The key proteins related to virulence and molecular networks that will be obtained in this study could provide new targets for the control of Verticillium wilt and new insights for the improvement of disease management.

大丽轮枝菌（Verticillium dahliae）引起的马铃薯黄萎病又称马铃薯早死病，是国内外马铃薯生产的重要限制因素。目前对大丽轮枝菌的致病机理缺乏深入了解，导致有效防治黄萎病面临的挑战愈发严峻。诸多研究表明，色素合成相关蛋白是大丽轮枝菌的毒力因子，但其调控大丽轮枝菌致病的机制尚不清楚。本项目以色素合成蛋白Vayg1参与的致病蛋白网络为切入点，拟通过蛋白组学技术分析野生型菌株和致病力缺陷型ΔVayg1突变体侵染马铃薯过程中的致病蛋白差异，研究Vayg1调控大丽轮枝菌致病的分子机理，以期鉴定和发掘相关致病通路，为全面解析黄萎病菌致病机制提供重要支撑。本研究获得的大丽轮枝菌关键致病蛋白和致病分子网络可为黄萎病防治提供新靶标，为改善病害防控措施提供新线索。

大丽轮枝菌引起的马铃薯黄萎病发生日趋严重，在世界范围内给马铃薯的安全生产带来了严重威胁。揭示大丽轮枝菌的致病机理对于开发黄萎病防治措施具有重要意义。前期研究表明，色素合成蛋白Vayg1对大丽轮枝菌的致病力具有重要作用，本研究在此基础上，采用蛋白组测序技术筛选出在大丽轮枝菌对马铃薯植株初侵染过程中受Vayg1调控的801个差异蛋白，其中包括4个关键候选致病蛋白VDAG_07165、VDAG_07742、VDAG_09909和VDAG_03665。通过生物信息学分析、基因功能评估、基因表达检测和酵母双杂交等研究表明，大丽轮枝菌的假定蛋白VDAG_07742仅参与致病过程，而假定蛋白VDAG_07165和Ca2+信号途径中的蛋白激酶C VDAG_09909在大丽轮枝菌生长发育和致病过程中均发挥了重要作用，黑色素合成途径中的关键催化酶VDAG_03665不仅参与大丽轮枝菌对马铃薯的侵染过程而且与Vayg1具有互作关系。以上结果为全面理解大丽轮枝菌致病机理提供了重要支撑，也为黄萎病防治开发了新靶标。