应用抑制消减杂交法克隆妊高征相关基因

Pregancy-induced hypertension(PIH) is one of the most important risk that involves in the mortality of the pregnancy women and fetuses. The mechanism of PIH is still unknown. Current studies suggested PIH is related with immunity, heredity and neuroendocrine. Objective Establishment of a profile in differential expressed genes in placental tissue from cases of pregnancy induced hypertension. Method The total RNAs were isolated from the tissues by Trizol and mRNA was purified by Midi Kit. The expression level of 17000 genes in placental tissue were evaluated by cDNA array technique. Results Three hundred and thirteen differentially expressed genes were identified in placental tissue of PIH compared with normal pregnancy cases. Two hundred and seventy four genes were up-regulated and thirty nine were down-regulated. There are twenty nine unknown EST fragments and sixty-two cDNA fragments among these differentially expressed genes. The other involve in transcription and translation, cell differentiation, receptor, apoptosis, immune, metabolism and growth. Conclusion A profile in differential expressed genes in placental tissue from cases of pregnancy induced hypertension has been established.

本项目以妊高征患者为研究对象,采用抑制消解杂交法研究妊高征患者与正常妊娠者胎盘组胁钜毂泶锏幕?并运用突变检测技术进一步论证这种基因的差异表达是否有基因调控区的突变所导致.揭示胎儿基因特异表达在妊高征发病中的作用,对阐明妊高征发病的分子机制具有重要意义.