水稻热激反应调控因子-HsHS的功能和机理研究

Heat stress is severely harmfulto the rice growth and production. A novel rice WRKY transcription factor (HsHS) has been identified to regulate programmed cell death induced by high temperature and to respond to several biotic/abiotic stresses and JA. These results imply that HsHS plays a crucial role in the heat shock response. HsHS may be a multi-functional factor, and play an important role in the disease resistance and/or other abiotic stresses too. In order to prove our hypothesis, and reveal the gene function and regulation mechanism, transgenic plants with depressed or overespressed HsHS gene were established, and the genes directly regulated by HsHS were being analyzed with RNA-seq and ChIP-seq, and the proteins that interacted with HsHS will be discovered by screening the Yeast Two-Hybrid library. The result will significantly contribute to further our knowledge about the rice responses to stress and rice improvement.

高温热害对水稻的危害非常严重。我们发现一个新的水稻WRKY转录因子-HsHS，调控高温诱导的细胞程序性死亡。另外，HsHS还受多种生物和非生物胁迫因子以及茉莉酸（JA）的诱导,这些暗示它可能是一个处于调控网络中关键节点的多功能转录因子，在水稻的抗病和抗逆反应中都起着重要作用。本研究拟以野生型、超表达和抑制表达转基因植株为材料，采用高温等环境逆境或不同病原菌接种处理,利用RNA-seq、ChIP-Seq和酵母双杂交等技术，揭示该转录因子的功能及其调控机制，研究结果对水稻热激等逆境反应机理的阐明以及水稻的改良具有重要的意义。

高温热害对水稻的危害非常严重。项目完成初步揭示了编码WRKY转录因子的OsHsHS基因的功能。超表达的OsHsHS转基因植株对热敏感，表现出早衰、不育或育性降低。多种生物和非生物胁迫诱导或抑制该基因的表达。OsHsHS基因的表达表现出较强的时空性，植株生长的早期表达较弱，而在成株期的叶片中表达很高，此时期的超表达转基因植株对热最敏感。通过RNA-seq分析，部分揭示了OsHsHS调控的基因。我们揭示了与OsHsHS互作的VQ蛋白质，重点研究了VQ8与OsHsHS之间的关系。结果表明，VQ8与OsHsHS的结合，抑制了OsHsHS与启动子的结合，从而改变了OsHsHS靶基因的表达。这些结果，加深了人们对OsHsHS对热调控机理的认知。