离子通道参与温度敏感神经元信号转导的分子机制

The molecular and cellular mechanisms by which the firing of temperature-sensitive neurons(TIN), the body of central thermoregulation induced by temperature is one of the focus of recent neuroscience research. Mainly dealing with all kinds of media levels under pathophysiological condition , previous study largely neglected the study of ion channel underly electrophysiological reactions. The project use patch-clamp and cytokine-related techniques to solve the problem and the main results are as follows:.(1). All sorts of hypothalamic TSN ion channels were considered, of which three channels to involved in signal transduction were dominant, including delay rectifier K+ channel (IK), L-Ca2+ and Ca2+ activated K+ channel (KCa). (2). The kinetic and pharmacological properties of TSN intrinsic ion channels were investigated. (3). The temperature-dependent properties of the above channels were studied. (4) During exploring the modulation of TSN ion channels signaling pathways, the close relationship between ion channel and oxidation、reduction and NO-cGMP signaling cascade was identified. Importantly, these results could be contribute to elucidating the mechanism of fever pathophysiology.

用膜片钳和动态单细胞Ca2+立体三维分布技术，在热敏（WSN）和冷敏（CSN）神经元上，研究电压依赖性和配体门控性离子通道的特性，探讨其温度依赖性的动作电位触发机制，观察内生致热原和细胞因子跨膜信号转导的离子通道机理，分析离子通道和G蛋白偶联第二信使低臣涞墓叵怠＝饩鲋惺嘈蕴逦碌鹘谟敕⑷炔±硌Щ浦?一些信号转导的关键性问题。