基于海马-穹窿周区orexin-A通路的胃俞募配穴对胃运动调节的机制研究

The preliminary results of this project indicate that Stomach Shu-Mu acupuncture points which regulates gastric motility demonstrate synergism. Acupuncture feedback at Stomach Shu-Mu acupuncture points converges and integrates at the Dorsal Vagal Complex (DVC) and the Paraventricular Nucleus (PVN), in return regulates gastric motility through the vagus nerve. The hippocampus plays a prominent role in gastric motility regulation as the autonomic integration center of the limbic system. On one hand, it receives afferent fibers of the splanchnic nerve. On the other hand, it has extensive fiber connections with the medulla oblongata, hypothalamus and amygdala-central components of gastric motility. This study elaborates on the relationship between the regulatory effects of Stomach Shu-Mu acupuncture point on Gastric motility and the hypothalamus. based on the relationship between the orexin-A signal pathway in the Hippocampal Fornix and gastric motility, Acupuncture at the Stomach Shu-Mu Points was combined with the hippocampal microinjection of orexin-A and its receptor antagonist (SB334867) to record the changes in gastric motility. At the same time, micro array electrodes were used to record the discharge of GD sensitive neurons and the expression of c-fos at the hippocampus, to preliminarily inspect the mechanism of hippocampal orexin-A pathway and Stomach Shu-Mu Acupuncture Points on gastric motility to provide an experimental basis for future studies on the Stomach Shu-Mu acupuncture points’ central action target and neural pathway related to their synergistic effects on gastric motility.

本项目组前期研究结果表明胃俞募配穴调节胃运动具有协同效应，且胃俞募穴针刺信息在延髓迷走孤束复合体（DVC）和下丘脑视旁核（PVN）汇聚整合，通过迷走神经参与对胃运动的调节作用。海马作为边缘系统的内脏整合中枢，在调节胃运动方面也起着重要作用。它一方面接受内脏神经的传入纤维，另一方面与延髓、下丘脑、杏仁核等与胃运动有关的中枢间存在广泛的纤维联系。本研究以胃俞募配穴调节胃运动与海马的关系为切入点，基于海马-穹窿周区orexin-A通路与胃运动的关系，在针刺胃俞募配穴基础上通过海马区微量注射orexin-A及其受体拮抗剂（SB334867）来记录胃运动的变化，并同时微阵列电极记录海马区GD敏感性神经元放电活动的改变、海马c-fos表达等，初步探察海马orexin-A通路与胃俞募穴对胃运动的调节作用机制，为进一步研究胃俞募穴对胃运动协同效应的中枢作用靶点和神经通路提供实验依据。

本研究以胃俞募配穴调节胃运动的中枢机制为切入点，开展针刺胃俞、募穴信息与边缘系统海马关系的机制研究，为胃俞募配穴协同效应的中枢作用靶点提供生物学依据。在课题组的前期研究基础上，本次研究探察海马orexin-A通路与胃俞募穴对胃运动的调控作用机制。主要研究内容与结果如下：①通过观察电针胃扩张模型大鼠胃俞募穴对胃内压、胃电的影响及海马c-fos表达、海马神经元放电情况，证明胃俞、募穴针刺信息在海马发生汇聚整合，边缘系统海马参与胃俞募穴对胃运动的调节作用。②通过观察电针胃扩张模型大鼠海马内微量注射orexin-A及其受体拮抗剂SB-334867对胃内压、胃电的影响及海马神经元放电情况，发现海马内注射orexin-A能增强大鼠胃运动，注射其拮抗剂后能够阻断此兴奋效应，而在拮抗剂存在的情况下，电针对胃运动的调节作用不明显，从而说明海马orexin-A通路参与了电针胃俞募穴对胃运动的调控。.本项目实施从orexin-A神经环路角度揭示胃俞募穴与胃运动的关系，为临床提供科学性依据，为后续基于神经环路研究胃俞募穴调节胃运动的中枢机制提供思路与奠定基础。