材料学因素对骨髓间充质干细胞凝集、分化及成骨模式的影响及调控

Bone formation takes place through two ossification modes: intramembraneous and endochondral bone formation. It mainly depends on if the mesenchymal cell differentiate into osteoblasts or chondrocytes, which would be regulated by the micro environment, growth factor and transcriptional factors. Our previous study demonstrated that the selection of the bone regeneration materials had a direct influence on the MSC differentiation lineage and the selection of the ossification mode. In this project, we examine the effect on MSC differentiation lineage, selection of ossification modes, under the regulation of the materials factors, to reveal if the MSC differentiation fate is regulated directly by the material itself or through its influence on the BMP regulation. We also intend to study the interaction of different growth factor with the materials and identify the potential materials factors that regulate the MSC condensation, differentiation, and the selection of ossifications modes. This study will not only greatly enhance our understanding of the signal pathways and molecular mechanisms of the osteogenesis and chondrogenesis during bone regeneration, but also provide valuable guideline in the selection and design of bone and cartilage repair and regeneration materials for clinical applications.

生物体内骨的发生有膜内成骨和软骨成骨两种方式，成骨模式的选择与干细胞选择向成骨或成软骨方向分化有密切关系，并受到微环境、生长因子、转录因子等因素调控。本研究小组前期研究表明骨修复材料的选择直接影响干细胞分化途径及成骨模式的选择。因此，并课题将深入研究人工合成骨替换材料作用下，干细胞分化命运及成骨模式选择的影响及调控机制，明确材料是直接影响抑或通过影响BMP对干细胞的调控来影响骨髓间充质干细胞的分化命运，揭示材料与不同生长因子（BMP、VEGF、TGF等）的交互作用，特别了解骨髓间充质干细胞的凝集对其分化途径的影响及材料学因素在其中所起的作用。该研究结果不仅能显著促进人工骨修复进程中成骨、成软骨进程的信号通路及分子机制的了解，也会进一步在临床应用中为骨和软骨修复材料选择及设计起到良好的指导作用。

生物体内骨的发生有膜内成骨和软骨成骨两种方式，成骨模式的选择与干细胞选择向成骨或成软骨方向分化有密切关系并受到微环境、生长因子、转录因子等因素调控。本研究小组前期研究表明骨修复材料的选择可能直接影响干细胞分化途径及成骨模式的选择。因此，并课题将深入研究人工合成骨替换材料介导下，干细胞分化命运及成骨模式选择的影响及调控机制，明确材料是直接影响抑或通过影响BMP对干细胞的调控来影响骨髓间充质干细胞的分化命运，揭示材料与不同生长因子（BMP、VEGF、TGF等）的交互作用，特别了解骨髓间充质干细胞的（募集）沉积对其分化途径的影响及材料学因素在其中所起的作用。该研究结果不仅能显著促进人工骨修复进程中成骨、成软骨进程的信号通路及分子机制的了解，也会进一步在临床应用中为骨和软骨修复材料设计及选择起到良好的指导作用。