基于电化学性能的呕吐毒素分子印迹聚合物的制备及其传感平台的构建

The pollution of Vomitoxin in cereal crops and feed is the serious challenge all over the world, therefore, it is very significance to establish a high sensitive, fast and simple molecularly imprinted electrochemical sensor to detect the vomitoxins. A kind of vomitoxin molecularly imprinted polymer with electron transfer properties will be synthesis in this project; and molecularly imprinted polymer sensing electrode will be study by ionic liquids based on the high viscosity and high electron transfer properties, which will increase the specificity and sensitivity of the sensor because of the electron of integrated of electron interface with the recognition element. including: 1、The template molecule of Vomitoxin will be isolated and purified from F. graminearum；2 A graphene quantum dots as the carrier with the electronic transmission performance and great specific surface area, molecularly imprinted polymers modified bare electrode wll be synthesized using surface polymerization method, which not only has specific identification, but also has excellent electrochemical performance; 3 Preparation of the molecularly imprinted sensing electrode with the integration of electrode interface and recognition element will be study. Molecularly imprinted electrode electrochemical performance of sensing electrode will be analysed using cyclic voltammetry, AC impedance and conductivity. The expected results of this project is to construct high sensitivity, strong specificity and good stability of Vomitoxin molecularly imprinted electrochemical sensing platform and applied to rapidly detect Vomitoxin on cereal crops, breakfast food and feed samples.

呕吐毒素在粮食作物和饲料中的污染是全球面临的严峻挑战，建立快速、简便和高灵敏的分子印迹电化学传感检测方法具有重要的意义。本研究拟合成一种具有电子传递性能的呕吐毒素分子印迹聚合物；然后利用离子液体的高粘度和优良的电子传递性能，制备一种传感电极界面和识别原件一体化的呕吐毒素分子印迹电极，提高传感器的稳定性和灵敏度。具体包括：1.从禾谷镰刀菌大麦固体培养物中分离纯化的呕吐毒素为模板分子；2.选用电子传递性能好、比表面积巨大的石墨烯量子点为载体，采用表面聚合方法，合成一种既具有分子印迹聚合物的特异识别性，又具有优良电子传递性能的裸电极修饰材料；3.研究传感电极界面和识别原件一体化分子印迹电极制备方法。采用电化学方法表征印迹传感电极的灵敏度、稳定性和重现性等参数。构建灵敏度高、响应速度快和稳定性好的呕吐毒素分子印迹电化学传感平台，用于粮谷类、早餐食品和饲料样品中DON的快速检测。

真菌毒素，是一类由真菌产生的有毒的次生代谢产物，其污染在全世界范围都非常普遍，给人类的健康和经济造成了一定的危害，因此，建立快速，灵敏，选择性强的检测真菌毒素新方法具有重要意义。本研究针对脱氧雪腐镰刀菌烯醇（Deoxynivalenol DON），俗称呕吐毒素和玉米赤霉烯酮（Zearalenone ZEA）建立了2种电化学传感检测方法。具体研究内容如下：.（1）本研究以DON为模板分子，筛选了L-精氨酸作为功能单体，在COOH-MWCNTs修饰后的电极表面通过电聚合法制备印迹电极。通过SEM、EDS、傅立叶变换红外光谱（FTIR）和电化学循环伏安法对P-Arg-MIP/COOH-MWCNTs印迹电极进行了表征。构建了DON分子印迹电化学传感器，P-Arg-MIP/COOH-MWCNTs印迹电极显示良好的导电性、高有效表面积和良好的类抗体分子识别能力和亲和力，以铁氰化钾溶液为探针，该方法检测线性范围0.1 - -70μM展示良好的线性关系，检测限为0.07μM，对小麦面粉实际样品的检测中得到的回收率令人满意，具有良好的可行性。.（2）基于铜基-有机金属框架（Cu-MOF）结合磁性Fe3O4-氧化石墨烯材料（Fe3O4-GO）制备了一种检测玉米赤霉烯酮（ZEA）的电化学传感器。本研究构建的传感器具有较大的表面积、优异的电子传递能力、良好的选择性、较好的重复性和重现性。在最优条件下对ZEA展现了较宽的线性范围0.03-9 μM，低的检测限0.0255μM。对玉米粉、大米粉和早餐燕麦加标回收检测ZEA，回收率在92.5-115.8%之间（RSD<5%），表明本方法可以用于谷物产品中分析检测ZEA。