网格蛋白介导的受体内吞在百脉根结瘤因子信号传递中的生物学功能和作用机制研究

The symbiotic interaction of rhizobia and legumes, results in the formation of nitrogen-fixing root nodules.Firstly, the surface cell receptor of the plant cell is needed to recognize the nodulation factor secreted by rhizobia and regulate the expression of the downstream gene of the receptor, along with the migration of the nucleus, the cytoskeleton Rearrangement, cell wall degradation, cortical cell division, leading rhizobia into the plant cells to form the infection line, and ultimately the formation of nodule. In this study, we focused on the function and mechanism of clathrin-mediated receptor endocytosis in the transmission of nod factor signaling. The aim of this study was to confirm the NFs and NFRs by immunofluorescence and immunoprecipitation. Body NFRs enter the cytoplasm from the cell membrane through the endocytosis of the receptor, and transfer the nod factor signal to the physiological processes of the downstream effector. Revealing the molecular mechanism of the rumen factor signaling in plant cells, and elucidating the early symbiotic relationship between rhizobia and legume.

根瘤菌与豆科植物能形成一种特殊的共生体根瘤，首先需要植物细胞表面受体识别根瘤菌分泌的结瘤因子，激活受体下游基因的表达调控，同时伴随着细胞核的迁移、细胞骨架重排、细胞壁的降解、皮层细胞的分裂，引导根瘤菌进入植物细胞形成侵染线，最终形成共生体根瘤。本课题围绕网格蛋白介导的受体内吞作用在结瘤因子信号传递中的功能和机制展开研究，拟以免疫荧光，免疫共沉淀等技术为手段证实结瘤因子NFs、结瘤因子受体NFRs通过网格蛋白介导的受体内吞作用从细胞膜进入细胞质，将结瘤因子信号传递到下游效应子的生理过程。揭示结瘤因子信号在植物细胞中传递的分子机制，阐明根瘤菌与豆科植物早期共生关系建立模式。

根瘤菌与豆科植物之间的互作导致共生固氮根瘤的形成。固氮根瘤的形成首先需要豆科植物细胞表面受体识别根瘤菌分泌的结瘤因子，从而激活受体下游一系列相关基因的表达调控。与此同时伴随着细胞核的迁移、细胞骨架重排、细胞壁的降解、皮层细胞的分裂等，使得根瘤菌进入植物细胞形成侵染线，最终形成共生固氮根瘤。本课题围绕网格蛋白介导的受体内吞作用在结瘤因子信号传递中的功能和机制展开研究，通过免疫荧光，免疫共沉淀等技术为手段证实结瘤因子NFs、结瘤因子受体NFRs及共生受体SymRK通过网格蛋白介导的受体内吞作用从细胞膜进入细胞质，将结瘤因子信号传递到下游效应子的生理过程。证实了百脉根衔接蛋白AP2与受体NFRs及SymRK之间存在相互作用。这些结果初步揭示了结瘤因子信号在植物细胞中传递的分子机制，阐明根瘤菌与豆科植物早期共生关系建立模式。