流式细胞分析分选技术的发展及在细胞凋亡研究中的应用

This project has developed the new hardware and software of flow cytometer. That is (1) Designed and manufactured automatical device of the charging delay time and real time display for flow cytometer cell-sorting. This design simplified the operation and raised cell-sorting efficiency, and the higher sorting recovery and sorting rate can be achieved, at the same time high sorting purity is secured. (2) Designed a low-voltage and low price charging circuit for flow cytometer cell sorter which is a good alternative to the import instrument which use high voltage one, especially to researcher who hopes to build his own personal instrument. (3) A new data analysis software was developed, which can fits and quantitatively calculates the DNA distribution ration of cells in apoptosis and calculates the correlation coefficiency of variations of multiple parameters of cells. This project has developed flow cytometer analyzing and sorting function and has important foreseeable application prospect in the biology and medical research.

发展流式细胞仪硬件和软件。开发流式细胞分选充电延时时间的自动设置和分选收获率自动测量技术。开发新的数据分析软件，显示细胞的真三参数立体分布图；计算细胞多参数变化间的相关系数；拟合计算凋亡细胞的DNA分布。利用这些新功能开展对细胞凋亡的多参数研浚教窒赴蛲龅幕啤Ｕ庑┕δ茉诠噬仙形醇ǖ溃舸葱孪钅浚兄匾τ们熬啊