咀嚼对抗颌骨骨质疏松力生物学机理的多层次研究

The mandibular osteoporosis is the local show of systemic osteoporosis in oral cavity and one of the most important diseases of the oral cavity concerned. Masticatory can promote the growth of mandibular and improve alveolar bone structure. The project will explore the mechanobiological mechanism of masticatory against mandibular osteoporosis at multiscale according to the animal experiment and construct its mathematical model. Rat animal models of the mandibular under different loading will be established by feeding different hardness food through diet of free and timing, and load index of chewing force, chewing frequency and so on will be tested. Effect of masticatory on mandibular will be observed by adopting biomechanics test, bone histomorphometry, cellular and molecular biology and etal detection technology at three levels of whole, tissue and cell to clarify the mechanobiological mechanism of masticatory against mandibular osteoporosis at multiscale. The correlation of quantitative equation between mandibular bone structure,osteoblast,osteoclast, biochemical regulatory factors, biomechanical regulatory factors and geometrical regulatory factors will be established based on the result of animal experiment,and then mathematical model of the mechanobiological mechanism of masticatory against mandibular osteoporosis will be constructed to study the effect of masticatory on mandibular at quantitative level and thus to further clarify the mechanobiological mechanism of masticatory against mandibular osteoporosis.The study will provide theoretical foundation and quantitative calculation method for masticatory against mandibular osteoporosis.So it will contribute to release the pain of patients with mandibular osteoporosis and reduce reduce the financial burden of patients.

颌骨骨质疏松是骨质疏松症在口腔局部的表现，是口腔领域被关注的重要疾病。咀嚼能够促进颌骨发育、改善牙槽骨结构。本项目拟通过动物实验多层次探索咀嚼对抗颌骨骨质疏松的机理，构建数学模型。采取自由和定时喂养不同硬度饲料的方式，建立颌骨不同载荷动物模型，检测咀嚼力、咀嚼频率等指标；采用生物力学实验、骨组织形态、细胞与分子生物学检测等技术，从整体-组织-细胞三个层次，观察咀嚼对颌骨的影响，在细胞分子水平上阐明咀嚼对抗颌骨骨质疏松的力生物学机理。以动物实验结果为基础，建立颌骨骨结构与成骨细胞、破骨细胞以及生物化学调控因子、力学调控因子和几何调控因子的定量关系方程，构建咀嚼对抗颌骨骨质疏松的力生物学数学模型，在定量层次研究咀嚼对颌骨的影响，进一步阐明咀嚼对抗颌骨骨质疏松的力生物学机理。本研究将为咀嚼力学疗法防治颌骨骨质疏松奠定理论基础并提供定量计算方法，为颌骨骨质疏松患者解除病痛和减轻经济负担作出贡献。

颌骨骨质疏松是骨质疏松症在口腔局部的表现，是口腔领域被关注的重要疾病。咀嚼能够促进颌骨发育、改善牙槽骨结构。本项目主要开展了如下研究：一、力学角度探索颌骨力生物学机理，进行了考虑载荷特性及骨骼力学性能特征的骨生物力学调控机理、动载荷作用下骨重建力学调控机制、咀嚼载荷对颌骨影响的动力学研究、振动载荷对骨骼细胞流体剪切力影响的流固耦合数值研究；二、细胞分子生物学角度探索咀嚼对骨质疏松颌骨影响的力生物学机理，开展了咀嚼载荷下颌骨力学信号传导的研究、增强咀嚼载荷对抗去势大鼠颌骨影响的研究，以及增强咀嚼载荷对去势大鼠不同牙位牙槽骨骨形态计量指标影响的研究；三、咀嚼载荷对骨质疏松颌骨影响的数值仿真研究；四、骨力生物学数学模型研究，提出了基于应力状态的细胞分子水平力生物学骨重建模型、基于力生长因子的力生物学骨重建模型；五、口腔振动仪的研制，设计了用全身振动仪、小型颌骨振动仪；还开展了基于径向曲线的颅面统计复原研究、口腔医用器件3D打印研究-基于生物力学原理的定制口腔医用器件3D打印技术研究、颌骨骨质疏松对口腔种植体影响的研究、动静耦合载荷对正畸牙移动影响的研究等。项目基于颌骨的骨力学适应性，从力学角度和生物学均开展了相关研究，并设计了两种口腔用振动仪。本研究将为咀嚼力学疗法防治颌骨骨质疏松提供指导，为颌骨骨质疏松患者解除病痛和减轻经济负担作出贡献。