交直流复合磁场对昼夜节律的影响及其与隐花色素的关系

The AC - DC hybrid magnetic fields, caused by the AC - DC parallel transmission lines or magnetic levitation train, has aroused widespread concern and worry on its effect on health. Cryptochrome proteins were proven a magnetoreceptor protein by many articles, and the free radical mechanism based on cryptochrome protein is currently widely accepted to explain the bioeffects of geomagnetic field and extremely low frequency magnetic field. Moreover, Cryptochrome is a key gene of circadian clock.But there are few reports about how the magnetic fields affect circadian clock by cryptochrome. It is well known that the disruption of cicadian clock affect human health.So,it is important to reseach how the AC - DC hybrid magnetic fields affect the cicadian clock and its mechanism,since there is cryptochrome genes in human genome. This project is designed to study the bioeffects and its mechanism of the AC - DC hybrid magnetic fields on circadian rhythms and its genes, to explore the interaction of mechanism between the the AC - DC hybrid magnetic fields and the free radical mechanism based on cryptochrome protein. It is believed that this project is important to explain the effect of the electromagnetic environment due to AC-DC parallel transmission lines or magnetic levitation train. And it would provide new ideas and help to study the bioeffects and its mechanism of the magnetic fields.

交直流并行输电走廊和磁悬浮列车周围的交直流复合磁场环境引起人们对复杂电磁环境的广泛关注和担心，研究这种复合磁场对人的影响和机制具有重要意义。隐花色素具有感应地磁场的功能，在自由基对机制中起着与磁场相互作用的关键因素。隐花色素也是生物钟昼夜节律的关键基因，但是磁场如何通过隐花色素影响生物钟的运行的报道很少。生物钟是影响人类健康的重要因素，并且人类也含有隐花色素基因，也具有感知磁场的能力。因此，通过基于隐花色素蛋白的自由基对机制研究交直流复合磁场对生物钟的影响及其机制成为非常迫切的问题。本项目以生物钟昼夜节律及其分子基础为研究对象，利用生理学及分子生物学等手段研究交直流复合磁场对昼夜节律的影响，探讨基于隐花色素蛋白的自由基对机制在交直流磁场生物钟效应机制中的作用。本项目将对阐明交直流并行输电和磁悬浮列车引起的电磁环境对健康的影响具有重要意义，为磁场生物学效应及其机制的研究提供新的思路和帮助。

交直流并行输电走廊和磁悬浮列车周围的交直流复合磁场环境引起人们对复杂电磁环境的广泛关注和担心，研究这种复合磁场对人的影响和机制具有重要意义。隐花色素具有感应地磁场的功能，在自由基对机制中起着与磁场相互作用的关键因素。隐花色素也是生物钟昼夜节律的关键基因，但是磁场如何通过隐花色素影响生物钟的运行的报道很少。生物钟是影响人类健康的重要因素，并且人类也含有隐花色素基因，也具有感知磁场的能力。因此，通过基于隐花色素蛋白的自由基对机制研究交直流复合磁场对生物钟的影响及其机制成为非常迫切的问题。本项目成功研制了交直流复合磁场实验装置两套，以小鼠胚胎成纤维细胞NIH-3T3和大鼠为试验对象，从细胞和动物两个层面研究了交直流复合磁场对昼夜节律因子基因转录和大鼠褪黑素分泌的影响，实验结果表明磁场能够影响昼夜节律因子的表达，但对褪黑素的影响不明显。本项目还从理论上分析了磁场对生物昼夜节律的影响机制，并通过量子生物学模拟隐花色素自由基对机制，建立相关函数计算自由基对量子产率。本项目对阐明交直流并行输电和磁悬浮列车引起的电磁环境对健康的影响具有重要意义，为磁场生物学效应及其机制的研究提供新的思路。