新疆"阴虚内燥型"围绝经期焦虑症主证调查和对其相关靶蛋白调控实验研究

Based on the background of Northeast Dryness Syndrome (NDS) , regarding serious emotional disorders during perimenopause anxiety related family and society in Xinjiang ethnic minority. （1）To determine the distribution and constituent ratio of in deficiency and endogenous dryness type in Xinjiang and illustrate characteristic background and theoritical basis of correlation and susceptibility between the major syndrome type and NDS, investagation about major symptomatology characteristics of"Yin deficiency and endogenous dryness type "perimenopausal anxiety in regional Han, Urghur and Kazak patients and the relationship with NDS will be undertook (2) The "Northwest Dryness Syndrome" prevention experience of legislation and "Qinghao Biejia decoction combined with Northwest Dryness Syndrome experience" as the experimental formula will be used to treat "Yin deficiency and dryness type" perimenopausal anxiety rats combined model. RT- PCR and Western blot will be applied to detect the AQP3mRNA and protein expression in rat model in order to identify the regulatory effect of Ziyin Runzao method and its representative prescription of "Yin deficiency and endogenous dryness type"perimenopausal anxiety, and to provide the experimental evidence for the establishment of prevention program of Northeast Dryness Syndrome in Xinjiang and emotion immprovement of patient.

基于"西北燥证"研究基础，针对新疆民族围绝经期焦虑症关联家庭社会的严重情绪障碍问题，（1）通过新疆汉维哈三个民族"阴虚内燥型"焦虑症主证调查及与"西北燥证"相关性调查，确定新疆民族围绝经期焦虑症患者主要证型即阴虚内燥证分布与构成比，分析阐明其与"西北燥证"相关易感性的背景特征和理论基础；（2）通过建立"阴虚内燥型"围绝经期焦虑症大鼠病证结合模型，以基于"西北燥证"防治经验的立法及"青蒿鳖甲汤合西北燥证经验方"为实验方剂，采用 RT- PCR 及Western blot 的方法，进行模型大鼠AQP3mRNA和蛋白表达的调控实验研究，探索验证AQP3为"阴虚内燥型"围绝经期焦虑症关键靶蛋白和"西北燥证"防治经验及其代表方剂对它的调控作用，为筛选建立新疆民族围绝经期焦虑症适宜性防治与推广方案，改善患者情绪障碍，提出实验依据。

项目背景与主要研究内容：在“西北燥证”研究基础上，本课题围绕“阴虚内燥型”围绝经期焦虑症主证和“青蒿鳖甲汤合西北燥证经验方”对其相关靶蛋白调控机制这一科学问题，开展临床典型病例调查和建立“阴虚内燥型”围绝经期焦虑症大鼠病证结合模型，进行模型大鼠AQP3mRNA和蛋白表达的调控实验研究，探索验证AQP3为“阴虚内燥型”围绝经期焦虑症关键靶蛋白和“西北燥证”防治经验及其代表方剂对它的调控作用。.本项目按计划完成了1036例典型病例调查和“青蒿鳖甲汤合西北燥证经验方”对“阴虚内燥型”围绝经期焦虑症大鼠模型调控实验。.重要结果及关键数据：典型病例调查研究发现：①“围绝经期焦虑症”与“绝经年限”，“初潮年龄”，“分娩次数”，“流产次数”有一定关联，“绝经年限”高，“初潮年龄”大，“分娩次数”，“流产次数”多，患围绝经期焦虑症风险增高；②“阴虚内燥证”是“围绝经期焦虑症”的主要证型，占“围绝经期焦虑症”中医证型的77.0%；③“围绝经期焦虑症”患者中兼有“西北燥证”的比例为78.6%，远高于健康对照，“西北燥证”可能是围绝经期焦虑症的危险因素。实验研究发现：①“青蒿鳖甲汤合西北燥证经验方”可改善阴虚内燥型“围绝经期”大鼠模型性激素水平；调控皮肤组织AOP3蛋白，增加AOP3蛋白表达量，从而影响“阴虚内燥型”围绝经期大鼠模型皮肤内环境，进而调节表皮通透屏障功能。②“青蒿鳖甲汤合西北燥证经验方”可改善“阴虚内燥型”围绝经期焦虑大鼠模型神经递质水平，从而改善阴虚内燥型“围绝经期”大鼠模型焦虑躁狂等症状。.科学意义：围绝经期焦虑症“阴虚内燥型”主证及其与“西北燥证”关联系研究，对利用“西北燥证”防治经验辨证论治“围绝经期焦虑症”提出客观依据，同时延伸和拓展了“西北燥证”研究的理论价值和临床指导性；实验研究发现对开发与推广“阴虚内燥型”围绝经期焦虑症适宜性防治方案提出科学依据。