聚乙烯撑碳酸酯类新型酶固定化载体的合成和性能

The monomer containing cyclic carbonate, vinylene carbonate, was prepared. Chain transfer reaction and aminolysis in homopolymerization were investigated to obtain the data of enzyme immobilization on crosslinked copolymer bead. Several kind of crosslinking agents were selected to use as enzyme support having various skelecton structures. The condition of aminolysis reaction between cyclic carbonate in polymer and amino-group was determined. The support was applied to immobilization of glucoamylase and trypin..Keywords Vinylene carbonate, aminolysis, enzyme support, glucoamylase, trysin.

聚碳酸乙烯撑酯分子链上的环碳酸酯基可在室温下与胺基反应形成氨基甲酸酯。通过采用碳酸乙烯撑酯与其它单体共聚或直接在含胺基的树脂上悬挂聚碳酸乙烯酯大分子，制备出含厶妓嵋蚁┏捧シ肿恿吹亩嗫捉涣靥澹糜诿傅壬锘钚晕镏使潭ɑ？梢栽ぜ迷靥褰且恢衷谟τ蒙暇哂蟹奖阈裕榷ㄐ院凸惴盒缘男滦凸潭ɑ锘钚晕镏试靥濉?.