改性合成石墨烯量子点在植物体上的传输机理、原位成像和示踪

Tracking and imaging of nanomaterials in vivo is of great importance for understanding its transport behavior and biotoxicity in the environment. Graphene quantum dots (GQDs), one novel species of ultrasmall graphene-based nanomaterials that possess exceptional physical and chemical properties, have inspired intensive research efforts since being synthesized very recently. With such promising potential for multiple applications, GQDs would probably be released into the environment and transported between environmental media, which may trigger uncertain ecological effects. Due to its small size, GQDs could be transported further and easier in the environmental media than those with large dimensions. Furthermore, it could damage the normal functioning of plant at cell or gene level. Therefore, the investigation of their transport and interaction with plant is needed. In the current study, N,S co-doped graphene quantum dots will be hydrothermally synthesized in a controllable way to achieve specific fluorescent properties. Photoluminescence that with high quantum yield and different from the autofluorescence of plant organism is expected. After been synthesized, N,S-GQDs will be used both as an auto-biomarker and carrier in its own imaging and tracking in the plant. The nanotoxicology of N,S-GQDs and the safety of its biomedicine application will be investigated.

纳米材料在植物体中的原位观测和示踪对了解其迁移转化行为及植物毒性机制具有重要的参考价值。作为一类具有发光特性的小尺寸二维碳质纳米材料，石墨烯量子点在生物标记、成像，药物载体等应用研究领域被寄予厚望，但由于尺寸小，迁移性强，传输深度大，可能会对植物体在细胞和基因水平上产生毒性，因此研究石墨烯量子点在植物体上的吸收传输过程对了解和预测其毒性机制，农产品安全等都具有重要意义。针对目前石墨烯量子点在植物体中原位观测和表征难以实现的问题，本项目拟对石墨烯量子点进行改性合成，得到一种或几种氮硫原子掺杂的石墨烯量子点；通过调控掺杂原子比例和合成条件等因素，期望获得具有较高量子产率，且荧光响应能够与植物体自身荧光进行区分的发光材料。进而原位观测掺杂石墨烯量子点自身及作为载体在植物体内的吸收分布及其与植物组织的相互作用，探讨协同作用机理，为正确评估其环境风险提供理论依据。