基于Notch信号通路与血管生成相关研究煨脓生肌法促进烧伤创面愈合的作用及分子机制

Simmer septic Shengji therapy is characteristic of TCM treatment of burn treatment, having made extensive and reliable curative effect in the clinical treatment, but the specific mechanism of simmer Septic Shengji therapy is not yet very clear for the treatment of burns. In previous studies, we found with the method of cubic alum ice microemulsions, at least by raising the epidermal growth factor (EGF) express this way, and achieve the purpose of promoting burn wound healing. Existing research shows that angiogenesis is a key of wound healing, at the same time, the Notch signaling pathway and angiogenesis in vascular development also plays an important role, but the correlation of the Notch signal pathway and angiogenesis in burn wound healing is not clear. As a result, we proposed the rabbit deep Ⅱ burn model to further study, through the detection of Notch signaling pathways, ligand and receptor downstream target genes and angiogenesis activation factor, the change of the suppressor gene and protein expression, to clear Notch signal pathway associated with angiogenesis in burn wound healing, and through the alum ice nano emulsion of interference from the Notch signal pathway, preliminarily clarify the specific targets for the treatment of burn and pathways, thus clear simmer Septic Shengji therapy of treating burn, for its widely used in clinical and provide a more solid theoretical foundation and scientific basis.

煨脓生肌法是中医治疗烧伤的特色疗法，在临床治疗中取得了广泛而可靠的疗效，但煨脓生肌法治疗烧伤的具体作用机制尚未十分明确。我们在前期研究中发现，以该法立方的矾冰纳米乳至少是通过上调表皮生长因子（EGF）表达这一途径，而达到促进烧伤创面愈合的目的。现有研究表明，血管生成是创面愈合的关键，同时，Notch信号通路也在血管发育和血管生成发挥极其重要的作用，但是Notch信号通路和烧伤创面愈合中血管生成的相关性还不清楚。因此，我们拟采用兔深Ⅱ 烧伤模型进一步进行研究，通过检测Notch信号通路受体、配体和下游相关靶基因及血管生成激活因子、抑制因子基因及蛋白表达的变化，明确Notch信号通路与烧伤创面愈合中血管生成的相关性，并通过矾冰纳米乳对Notch信号通路的干预，初步阐明其治疗烧伤的具体作用靶点和作用途径，从而明确煨脓生肌法治疗烧伤的分子机制，为其在临床的广泛应用提供更为坚实的理论基础及科学依据。

煨脓生肌法是中医治疗烧伤的特色疗法，在临床治疗中取得了广泛而可靠的疗效，但煨脓生肌法治疗烧伤的具体作用机制尚未十分明确。我们在前期研究中发现，以该法.立方的矾冰纳米乳至少是通过上调表皮生长因子（EGF）表达这一途径，而达到促进烧伤创面愈合的目的。现有研究表明，血管生成是创面愈合的关键，同时，Notch 信号通路也在血管发育和血管生成发挥极其重要的作用，但是Notch 信号通路和烧伤创面愈合中血管生成的相关性还不清楚。因此，我们拟采用兔深Ⅱ°烧伤模型进一步进行研究，通过检测Notch 信号通路受体、配体和下游相关靶基因及血管生成激活因子、抑制因子基因及蛋白表达的变化，发现Notch 信号通路烧伤创面愈合中血管生成存在一定的相关性，Notch信号通路被抑制，则创面愈合率下降，血管生成因子VEGF、VEGFR2表达下调，而血管抑制因子MMP2、MMP9表达上升。而以煨脓生肌法立法的矾冰纳米乳对烧伤创面愈合的机制也可能是与其Notch 信号通路受体、配体及靶基因的表达，从而干预了血管生成因子的表达有关，初步阐明其治疗烧伤的具体作用靶点和作用途径，也明确煨脓生肌法治疗烧伤的可能的分子机制，为其在临床的广泛应用提供更为坚实的理论基础及科学依据。