双发射量子点比率型荧光分子印迹传感器的制备及其对磺胺类抗生素药物的选择性识别与可视化检测性能研究

Sulfonamide antibiotics are widely used in treating infections in livestock production. Due to their excessive and injudicious use, sulfonamides not only influence of ecological environment, but also may endanger human body health. Therefore, the method with high selectivity, sensitivity, and photostability is established to detecting sulfonamides residue is one of the hotspot to solve the problem of environment. This project will focus on the advantages of the molecular imprinting technology, polymer polymerization method, and ratiometric fluorescence detection technology. The key of this project is the combination of the molecular imprinting selective recognition technology and fluorescence recognition technology. Herein, we first design a novel molecularly imprinted dual-emission ratiometric fluorescent sensor by using two different colored quantum dots as the fluorescent materials and molecular imprinting technique. The synergetic relationship of the physicochemical property and performance of the designed material will be researched. By combining fluorescence analysis method and visual detection technology, a new method for the selective recognition and visual detection of sulfonamides will be developed and applied to the fast and efficient determination of pyrethroid sulfonamides.

磺胺类抗生素广泛应用于预防和治疗食源性动物疾病，经历长期累积严重影响生态环境，威胁人类健康，因此建立快速、灵敏和选择性分析检测磺胺类抗生素残留的方法是解决环境问题的热点之一。本项目将集中分子印迹技术、高分子聚合方法、比率荧光检测技术的优点，重点耦合荧光识别与分子印迹选择性识别技术，利用两种不同荧光颜色的量子点为荧光材料，结合分子印迹技术，创新性地设计合成双发射量子点比率型分子印迹荧光传感器，系统性地研究其理化性质与性能之间的协同关系，结合荧光分析法和可视化检测技术，建立选择性识别与可视化检测磺胺类抗生素残留的新方法，实现多种样品中痕量/超痕量磺胺类抗生素残留的快速和高效分析。

磺胺类抗生素广泛应用于预防和治疗食源性动物疾病，经历长期累积严重影响生态环境，威胁人类健康，因此建立快速、灵敏和选择性分析检测磺胺类抗生素残留的方法是解 决环境问题的热点之一。本项目以绿色CdTe量子点、红色CdTe量子点、ZnO量子点、Mn掺杂ZnS量子点、碳量子点为荧光载体，将分子印迹技术分别与反相微乳聚合技术、沉淀聚合技术、表面接枝共聚技术耦合制备了绿色CdTe量子点分子印迹传感器、红色CdTe量子点分子印迹传感器、绿/红-CdTe 比率型双发射分子印迹传感器、环境友好型ZnO量子点分子印迹传感器、Mn掺杂ZnS磁性量子点分子印迹传感器、碳量子点分子印迹传感器，采用透射电子显微镜（TEM）、傅立叶变换红外吸收光谱仪（FT-IR）、扫描电子显微镜（SEM）、荧光分光光度计等多种测试手段研究了上述量子点分子印迹荧光传感器的形貌、结构、组成和光学性能；结合荧光识别实验，研究了五种量子点分子印迹荧光传感器对目标物分子磺胺类抗生素的光学检测行为。结果表明上述五种量子点分子印迹传感器对磺胺类抗生素中的磺胺二甲基嘧啶具有较好的选择性和可视化识别性，并且能够实现在实际水样或牛奶样品中痕量/超痕量磺胺类抗生素的快速和高效检测分析，相关的荧光淬灭机制经过研究表明应该遵循的是光诱导电子转移荧光淬灭机制，最终建立了选择性识别和可视化检测磺胺类抗生素残留的新方法。