脱细胞脂肪组织基质与脂肪干细胞联合移植，促进创面愈合的研究

Stem cell therapy brings new hope into the repair of chronic and refractory wounds and large skin defects. In conventional methods of cell transfer, cells are directly injected into diseased target tissue. Injuries during the injection process combined with ischemia and inflammation in the microenvironment, compromise cell survival rate and reduce treatment efficacy. Co-transplantation with scaffold materials could effectively solve this problem, and the search for an easily accessible and clinically applicable scaffold has become the focus of recent researches in this field. Extracellular matrix (ECM) plays important roles in the regulation of cell proliferation, differentiation, and gene expression. Its three-dimensional structure closely resembles that of natural tissues. As supportive scaffolds, it possesses good biocompatibility and high induction potential. Decellularized adipose tissue (DAT), or adipose-derived ECM, accommodates ASCs in a friendly microenvironment, and promotes ASCs' adipogenetic and angiogenetic potential. Current researches are mainly limited to its use in adipose tissue engineering. Reports on DAT in wound healing are lacking. Based on our previous studies of ASCs and wound healing, our group plans to seed DAT with ASCs, and to determine the effects of 3D culture in DAT on ASCs' biological functions. In a nude mice full-thickness skin defect model, we would also explore the influence of DAT-ASCs co-transplantation on wound healing, and clarify the underlying mechanisms. ASCs are easily accessible, with abundant reserve in the human body. Our study will provide novel insights in stem cell therapy of refractory wounds.

干细胞为难愈性创面的修复带来了新的希望。传统方法将细胞直接注射到病变组织，注射造成的细胞损伤，微环境中的缺血和炎症可导致细胞存活率降低和疗效不佳。与支架材料共移植可有效解决这一问题。寻找一种获取简便且临床可用性强的支架材料是这一领域的热点。细胞外基质(ECM)对细胞的增殖、分化和基因表达发挥重要的调控作用，且与天然组织三维结构相似，广泛应用于组织工程研究领域。脱细胞脂肪组织基质(DAT)是脂肪来源的ECM, 具有促脂肪新生和促血管新生作用，可为脂肪干细胞(ASCs)提供良好的微环境。目前DAT在脂肪组织工程方面研究较多，在创面愈合领域尚未见报道。本研究在我们前期关于ASCs与创面愈合研究的基础上，将ASCs接种于DAT，确定与DAT共培养对ASCs生物学性能的影响，并观察DAT-ASCs共移植对创面愈合的影响。脂肪组织来源广泛，获取简单，本研究将为难愈性创面的干细胞治疗提供新的思路。

干细胞为难愈性创面的修复带来了新的希望。寻找一种获取简便且临床可用性强的支架材料是这一领域的热点。脱细胞脂肪组织基质(DAT)是脂肪来源的细胞外基质(ECM), 具有促脂肪新生和促血管新生作用，可为脂肪干细胞(ASCs)提供良好的微环境。目前DAT在脂肪组织工程方面研究较多，在创面愈合领域尚未见报道。本研究在我们前期关于ASCs与创面愈合研究的基础上，将ASCs接种于DAT、确定与DAT共培养对ASCs生物学性能的影响并观察DAT-ASCs共移植对创面愈合的影响。脂肪组织来源广泛，获取简单，本研究将为难愈性创面的干细胞治疗提供新的思路：我们获取健康女性脂肪抽吸物，分离得到DAT和ASCs,体外实验进行DAT-ASCs共培养，确定ACSs与DAT共培养比单纯培养基培养具有更高的增殖率并分泌更多的生长因子；同时我们还建立裸鼠创面模型，发现DAT-ASCs组的创面修复显著优于sham组、单独ASCs组和单独DAT组。因此，DAT ASCs 联合应用可为细胞移植提供有效的支撑和保护，为细胞的黏附和增殖提供了良好的三维生存环境，并因此对创面愈合起到促进作用。