TGF-β3对牙髓干细胞成骨向分化促进种植体周围骨整合的作用及其机制研究

Insufficient bone and long healing time in implant denture repair have long been a dragging issue for clinical efficiency. Attempts to apply cytokines and seed cells in clinical research have been a hot topic. Transforming Growth Factor-β3 (TGF-β3) could induce mesenchymal stem cells differentiation and Osteoblast proliferation. Dental Pulp Stem Cells （DPSCs） are multipotent adult stem cells originated from mesenchymal stem cells which can differentiate into variety of cell types. However, a combination of both induced osteogenesis mechanism is not clear. Our previously study had demonstrated that TGF-β3 could induced DPSCs into dentin, neural tissue, and directed differentiation to osteoblasts (OBs), accelerating the ossification process of the Guided Bone Regeneration (GBR) techniques. Related results had been published and no similar articles reported by others. Therefore, we hypothesis that exogenous TGF-β3 combined with DPSCs mediated by SMAD pathway can differentiate into bone tissue through stimulates endogenous TGF-β3, shortening implant osseointegration time by enhancing the osseointegration through accelerating the proliferation of osteoblasts. Our hypothesis shall be validated at cell-protein-gene-level by using IHC, q-PCR, Western blot, SEM and other technologies both in vitro and in vivo. This study will provide new ideas for implant-based denture repair and opens up new perspectives to reduce implant treatment cycle.

种植义齿修复中骨量不足、愈合时间长一直是困扰临床疗效的问题，TGF-β3可诱导间充质干细胞定向分化和调节成骨细胞的增殖；DPSCs是间充质来源成体干细胞，但二者结合诱导成骨的机制尚不明确。我课题组常年致力于TGF-β3对DPSCs定向分化的研究，前期研究已经证实TGF-β3可以诱导DPSCs向类牙本质转化，向神经组织转化，并且向OBs定向分化，加速GBR技术的成骨化过程。相关成果已发表，未见类似文献报道。我们假设TGF-β3结合DPSCs通过smad通路介导，可定向分化成骨，在体内外源性TGF-β3刺激产生内源性TGF-β3，加速成骨细胞增殖，进而增强骨整合的能力，缩短种植体骨整合的时间。我们拟通过体外体内研究，应用IHC、q-PCR、western blot、SEM等技术，从细胞-蛋白-基因水平，多角度验证我们的假说，此研究将为种植义齿修复提供新的思路。

种植义齿修复中骨量不足、愈合时间长一直是困扰临床疗效的问题，TGF-β3可诱导间充质干细胞定向分化和调节成骨细胞的增殖；DPSCs是间充质来源成体干细胞，但二者结合诱导成骨的机制尚不明确。我课题组常年致力于TGF-β3对DPSCs定向分化的研究，前期研究已经证实TGF-β3可以诱导DPSCs向类牙本质转化，向神经组织转化，并且向OBs定向分化，加速GBR技术的成骨化过程。相关成果已发表，未见类似文献报道。我们利用TGF-β3结合DPSCs通过smad通路介导，可定向分化成骨，在体内外源性TGF-β3刺激产生内源性TGF-β3，加速成骨细胞增殖，进而增强骨整合的能力，缩短种植体骨整合的时间。我们通过体外体内研究，应用IHC、q-PCR、western blot、SEM等技术，从细胞-蛋白-基因水平，多角度验证我们的假说，此研究将为种植义齿修复提供新的思路。