基于纳米半导体表面光敏蛋白复合膜的原位光电化学分析

Among the proteins with the photoelectric function, the photosynthetic reaction center (RC) isolated from Rb. Sphaeroides is the most attractive one. When the RC is excited by light, it converts efficiently the light energy to chemical energy, meanwhile, a series of charge separation and electron transfer (ET) happen. The cofactors in RC participate jointly in the complicated and photoinduced multi-routes ET processes. The studies of the photoinduced and electric-field-driven ET processes are very important for revealing the mechanism of electron transfer and energy transfer and building up a mimic photoelectric element on the molecular level. The studies for Rb. Sphaeroides, especially for the ET processes in RC, are more attractive and hundreds of papers are published every year. However, most of the studies on the photoelectric processes are performed by the spectral and biological methods and are indirect. Moreover, many problems associated with the photoelectric reaction parameters and the ET mechanism are still unsolved. In this project, the investigations surrounding RC were carried out with the electrochemical and photoelectrochemical methods, and a series of the innovative results were obtained. This research was of great significance for further understanding the ET processes and the photoelectric conversion mechanism in RC, fabricating the mimic biophotoelectric element and optimizing the performance of the biophotoelectric element.

利用现场快速光谱结合电化学仪对细菌光敏蛋白敏化的纳米半导体复合膜的光电化学行为进行原位分析。通过对该功能膜内光诱导或电驱动引发的电子传递和物种转换过程的研究，阐明光电化学反应涉及的复杂电子转移机理，同时探索光敏蛋白质的分析新手段。该课题的研究有助于人工蛋白质膜功能实现过程的表征，为分子水平光电器件研制奠定基础。