天然绿色家蚕丝对皮肤光氧化损伤的防护作用及机制

Naturally green-colored domestic silk has been found to be the most active antioxidant fiber. Due to the fact that oxidative damage is one of the main facts for light-induced injury in human skin, thus it could be a promising candidate for developing antioxidant textiles for protection of skin from routine light-oxidative injury. In this research scheme, in vitro skin test is employed to study the transfer characteristics of the antioxidant substances from green-colored silk to human skin and the dose-effect relationship of the inhibition against oxidant stress in skin. UV-induced skin injury in mice is taken as an injury model, the protection mechanism of green-colored silk toward.oxidative skin injury induced by UV radiation will be elaborated. The effects of conditions of textile processing on the structure and activity of the antioxidant components will be also investigated . Accomplishment of this research could lay a theoretical and technical foundation for evaluating the effectness of bioactive textiles, as well as for development of naturally green-colored silk and other antioxidant materials for protection textiles for oxidative skin.

天然彩色家蚕丝绿色品种是我们前期发现的、到目前为止具有最高抗氧化活性的纤维。光氧化损伤是许多皮肤疾病的诱发因素，因而天然绿色家蚕丝纺织品在防护日常光氧化损伤所导致的皮肤疾病方面具有重要价值。本项目旨在明晰天然绿色家蚕丝防护皮肤光氧化损伤的发挥规律与机制，具体地：建立评价方法，研究天然绿色家蚕丝织物中抗氧化成分向皮肤组织迁移的性质；利用动物实验，研究抗氧化主要成分对皮肤光氧化损伤的防护作用与机制；研究固胶加工对纤维中抗氧化主要成分结构和活性的影响规律。本研究可为生物活性功能纺织品的效能评价提供借鉴，为开发和应用天然绿色家蚕丝等皮肤氧化损伤防护功能纺织品奠定理论基础。

光氧化损伤是许多皮肤疾病的诱发因素，具有优异抗氧化性能的天然绿色家蚕丝在防护日常光氧化损伤所导致的皮肤疾病方面具有重要价值。本项目通过Franz扩散池法研究了天然绿色蚕丝抗氧化组分经皮渗透的性质，获得了抗氧化组分经皮渗透受皮肤环境影响的规律、抗氧化组分在各皮层的分布规律及皮层中抗氧组分的量效关系；通过动物试验明晰了天然绿色蚕丝纤维及其抗氧化成分对皮肤光氧化损伤的抑制与修复作用及生化机制；通过细胞实验明了了天然绿色蚕丝抗氧化组分对光氧化损伤皮肤细胞的增殖、凋亡和相关蛋白表达水平的作用及调控机制；获得了天然绿色蚕丝颜色及抗氧化活性在常规制丝加工及产品使用条件下的变化规律，开发了一种基于环氧树脂化学交联固胶以提升天然彩色丝颜色及抗氧化性能稳定性的技术，获得了交联固胶固色的工艺参数。研究结果可为天然绿色蚕丝及其抗氧化提取物用于皮肤光氧化损伤的防护与修复奠定理论基础，为天然彩色蚕丝产品的加工与开发提供技术指导。