油菜种质资源抗旱性鉴定技术体系构建及耐旱分子机理解析

Rapeseed, as an important oil crop, is inclined to be subjected drought injury due to its relatively sensitive to drought stress among the primary crops. Therefore, it is of great significance for the screening and evaluation of the drought-resistant rapeseed germplasm, and the breeding of drought-resistant varities to construct the identification system of drought resistance and study the mechanism of drought-resistant in Brassica napus L..To research into the biological process of drought stress at different stage of rapeseed growth in a systematic way and establish rapid, accurate, simple and practical screening and identification technique system for drought-resistan rapeseed germplasm resources, the approaches of physiological and biochemical analysis, agronomic traits investigation, combining far IR thermography and chlorophyll fluorescence imaging will be employed. At the same time, solution culture, potted experiment and field drought treatment will be carried out. Using the screening and identification technique system, some drought-resistance rapeseed germplasm resources would be obtained. On that basis of the results above, drought-resistant and drought-sensitive varieties will be used to further analyze. The mRNA expression types and levels of transcriptional profile in the drought stress and control would be obtained by the technique of RNA-seq. Spatial-temporal and expression profiles of drought-related candidate genes of rapeseed could be screen out by microarray and bioinformatics analyses. Furthermore, we are going to construct regulatory network of drought-related genes and explore the key genes response to drought, reveal the drought-resistance mechanism of rape using comparative genomic and bioinformatics analysis

油菜作为重要的油料作物，在主要农作物中抗旱性较差，极易遭受干旱的伤害。因此，构建油菜抗旱性鉴定技术体系，研究油菜的耐旱机理，对于油菜抗旱种质资源筛选评价及抗旱品种培育具有重要意义。.本研究运用生理生化分析、农艺性状考查与远红外热成像、叶绿素荧光成像技术相结合，水培、盆栽与田间干旱处理相结合的方法，系统研究油菜不同生育时期干旱胁迫的生物学过程，建立快速、准确、简便、易行的油菜抗旱种质资源的筛选、鉴定技术体系，筛选获得耐旱种质资源。在此基础上，以筛选出的耐旱和旱敏感品种（系）为材料，运用转录组测序（RNA-seq）技术分析干旱诱导和非诱导胁迫处理的转录组表达信息，获得mRNA基因的表达类型及其丰度信息，通过基因芯片杂交及生物信息学分析，获得油菜抗旱相关候选基因的时空表达谱，并通过比较基因组和生物信息学分析，构建油菜干旱相关的基因调控网络，发掘油菜干旱响应的关键节点基因，揭示油菜的抗旱机理。

油菜作为重要的油料作物，在主要农作物中抗旱性较差，极易遭受干旱的伤害。因此，构建油菜抗旱性鉴定技术体系，解析油菜的耐旱分子机理，对于油菜抗旱种质资源筛选评价及抗旱品种培育具有重要意义。.本研究运用生理生化分析、农艺性状考查与远红外热成像、叶绿素荧光成像技术相结合，水培、盆栽与田间干旱处理相结合的方法，系统研究油菜不同生育时期干旱胁迫的生物学过程，建立快速、准确、简便、易行的油菜抗旱种质资源的筛选、鉴定技术体系，筛选获得35份全生育期综合抗旱性较好的种质材料。在此基础上，以筛选出的耐旱和旱敏感品种（系）为材料，运用转录组测序（RNA-seq）技术分析油菜在干旱胁迫下基因表达的动态变化以及抗旱和旱敏感品种中基因表达的差异；通过比对和软件分析，获得有pathway注释的差异基因总数为6440，进一步分析得到169个表达差异明显的基因，并对这些基因参与的代谢通路进行了分析，构建了代谢调控网络；同时对其中的22个表达差异基因，构建植物表达载体，并进行了油菜和拟南芥的转化。这些结果为进一步挖掘油菜抗旱基因及揭示抗旱分子机制奠定了坚实的基础。