多价抗前列腺癌/抗人CD3双特异单链抗体的构建及表达

To construct anti-CD3×anti-prostate-cancer bispecific single-chain antibody(BsAb) and the fusion gene of human IgG3 upper hinge region and p53 tetramerization domain respectively. Then the genes mentioned above were sequentially cloned into pUC18 vector to construct multivalent BsAb which can self-assemble tetramerization. It will exhibite an enormous gain in affinity. Successful construction of the fusion gene of human IgG3 upper hinge region and p53 tetramerization domain lays the foundation for further preparation of multivalent gene engineering antibody and break a new path to the improvement of antibody's affinity

本研究在已经构建抗人CD3单链抗体的基础上，利用人转录因子P53的四价功能域可自动装配成四聚体形式这一功能，通过基因工程技术构建人源化八价抗人前列腺癌／抗人CD3特异性チ纯固澹竦酶咔缀托缘耐笔侗餞淋巴细胞和前列腺癌细胞的双特异抗体，增加肿瘤部位T淋巴细胞的数量，并启动T细胞产生毒性效应，杀伤前列腺癌细胞。