活性聚合物支架与转基因骨髓基质细胞的成骨作用研究

Scaffolds is the primary factor of bone tissue engineering, and has an important biologic significance, because it can attach and induce seed cells into the specific cells and be transplanted.together into vivo.The ability is owed to its interspace construction. So the scientific interspace construction can prevent some factors which cann’t form bone, when these factors will enter into.scaffolds.By which it guarantees continuous and effective bone forming.Presently, the techinique.of making scaffolds has some shortcomings,as follow: Firstly, organosolvent what is remained in scafflods damages the activity of cells and cytokines; secondly, hyperthermia plucking does some lesions to molecular stability of scafflods, in the mean time, adds the difficulty and cost of smearing technique; thirdly, scaffolds is small and.thin, the holes in scaffolds are narrow and uneven.which isn’t benefited to osteoblasts’ attachment, proliferation and migration; fourthly, for inherent stagnant effect, the rate of forming holes in the procedure of making scaffolds is low, which affects that cells migrate into the interior of scalffods,and impede the change of metobolites; fifthly, scaffolds leash active cytokine unsteadily.To search for a novel technique on fabricating poly(lactide-co-glycolide) copolymer foamy.scaffolds that have better three-dimensional microstructure and suit for tissue engineering usage as.matrix material. Study the degradation properties of scaffolds, and ethanol disinfection effects on.its physical properties. Modify the material surface function and improve the hydrophilic ability.Construct cytokine control-release system in foamy scaffolds that sustained release growth factors..Join the conductive and inductive approaches together, thus the utilized basic of tissue engineering.scaffolds put into use prepared for orthopaedics in future..

采用低热黏结沥取+高压气体法制作三维结构精确控制的骨组织工程聚合物支架，ρ阅芪定。改性后具有高度亲嗽和持型放活性能力。体外培亚髓皇细阿叫BMP-7基因转导后分泌碱粤姿崦改芰ο灾岣撸牖钚跃酆衔镏Ъ芨春虾笥糜诠侨彼鹬瘟蒲芯浚銮砍晒亲饔谩＝亲橹こ倘蠡方谟谢岷希诜肿由镅教教钟行С晒欠椒?