基于能量转移的新型长荧光寿命有机荧光染料及纳米材料的构建及成像研究

Organic fluorescent dyes and nano materials with long fluorescence lifetime play important roles in time-resolved fluorescence imaging technology. Energy transfer systems are widely used because they can enlarge the Stokes shift of dyes. In traditional energy transfer systems, the disadvantages of donors and acceptors with short fluorescence lifetime ristrict the applications of them. Based on the mechanisms of FRET and TBET, our application will introduce coumarin and naphthalimide dyes as energy donors and the previous DCF-MPYM dyes with long fluorescence lifetime as energy acceptors. And we will construct two categories of multiple fluorescent dyes through changing the linkers between donors and acceptors. It will explore a new method for designing and synthesizing novel long fluorescence lifetime dyes and removing the interference brought by background fluorescence in duplicate ways. Meanwhile, nano silica particles can isolate the influence of outside oxygen by coating fluorescent dyes. The nanoparticles are the ideal protection matrix for fluorescent dyes beacause of its optical transparency and biological compatibility. Series of DCF-MPYM dyes will be introduced as energy acceptors, coumarin and naphthalimide dyes as energy donors, and will be coated inside silica nanoparticles to construct energy transfer fluorescent nano materials with long fluorescence lifetime. It will creat important opportunities for realizing more effective fluorescence imaging.

长荧光寿命有机荧光染料和纳米材料在时间分辨荧光成像技术中起着关键作用；能量转移体系能增大染料Stokes位移，故该系列染料被广泛应用。但传统能量转移体系供体和受体荧光寿命短的缺点，难以更好地显示能量转移体系的优势。本申请基于FRET和TBET机理，拟选择香豆素和萘酰亚胺类染料作为能量供体，以前期研究的长荧光寿命DCF-MPYM系列染料作为能量受体，通过改变供体与受体间的连接臂，构建两大类多个荧光染料，拟为设计合成新型长荧光寿命荧光染料和实现双重方式扣除生物背景荧光干扰影响探索一条新途径。同时，二氧化硅纳米颗粒包覆荧光染料能隔绝外界氧气对染料的影响，且其本身具有光学透明性和生物兼容性，将其选作染料理想的保护基质，拟将DCF-MPYM系列染料作为能量受体，香豆素和萘酰亚胺类染料作为能量供体包覆于二氧化硅纳米颗粒内，构建两类新型长荧光寿命能量转移荧光纳米材料，为实现更高效的荧光成像技术创造条件。

长荧光寿命有机荧光染料和纳米材料在时间分辨荧光成像技术中起着关键作用；能量转移体系能增大染料Stokes位移，故该系列染料被广泛应用。但传统能量转移体系供体和受体荧光寿命短的缺点，难以更好地显示能量转移体系的优势。基于上述研究背景，项目围绕构建两大类基于FRET和TBET机理调控的长荧光寿命的荧光染料和荧光纳米材料作为主要研究对象，通过不同的构筑方法以期获得染料体系的供体和受体染料，最终制备具有能量转移体系的染料，基于项目构筑的染料，实现了对生物医用材料、有毒小分子和蛋白质纤维材料的染色和检测；利用香豆素、萘酰亚胺以及偶氮类染料为母体，成功制备非常具有潜在应用价值的染料，为未来设计合成新型长荧光寿命荧光染料和实现双重方式扣除生物背景荧光干扰影响探索一条新途径，项目基本完成了申请书的预定目标。在此项目的资助下，取得了系列创新性的基础研究成果，在ACS Sustainable Chem. Eng.（1篇）、Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy （1篇）、J. Fluoresc. （1篇）和ChemistrySelect（1篇）等学术期刊发表SCI论文6篇，2篇中文核心期刊，授权2项中国专利，项目已完成研究目标，但是制备荧光纳米材料还有一定的难度，需要进一步的尝试。