表面功能调控的石墨烯三维组装体构建生物传感

Graphene has many unique properties and a broad prospect in electronics, energy, catalysis and sensing and other fields. But strong stacking effect between graphene layers led to decreased performance such as specific surface. Therefore, this project aims to prepare stable three-dimensional assembly structure of graphene, and to explore its application in biological analysis and sensing. First，we will prepare stable three-dimensional assembly structure of graphene by self-assembly method, hydrothermal method and organic gel-sol method, and change the property of graphene surface by connection of different types of functional group; Subsequently, enzymes and proteins will be immobilized on the three-dimensional assembly structure of graphene. We will study the electrochemical and electrocatalytic performance from biomolecules immobilized on the different interface. On this basis, construct the electrochemical biosensor based on the three-dimensional porous graphene assembly.

石墨烯具有诸多独特的性质，在电子、能源、催化和传感等领域有广泛的应用前景。但石墨烯片层间的强π堆积效应将使其诸多性能下降。为此，本项目拟制备具有稳定三维结构的石墨烯组装体，并探索其在生物电分析和传感中的应用。首先以模板自组装法、水热法和有机凝胶-溶胶法等制备具有稳定三维空间结构的石墨烯组装体，并结合化学修饰技术在石墨烯表面连接上不同类型的功能团；随后，将三维结构石墨烯组装体作为酶和蛋白质等生物分子固定化的基底，研究固定化生物分子电化学和电催化性能等；在此基础上，构建基于三维多孔石墨烯组装体的电化学生物传感器。

在项目资助的支撑下，建立了模板法制备了超轻多孔的氮掺杂石墨烯的方法；提出了金属纳米粒子-石墨烯复合材料的制备方法以及室温电化学方法制备氮掺杂石墨烯材料，研究了复合材料的电催化性能；提出了以聚赖氨酸为分散剂，用非共价法修饰石墨烯制备聚赖氨酸-石墨烯（PLL-G）杂化物，并利用聚赖氨酸的生物相容性和石墨烯的导电性，将其作为负载血红蛋白的介质，构建了电化学生物传感器。通过努力，已顺利完成，取得了系列性的研究成果，发表与录用SCI论文共8篇，获得授权发明专利1项。