高频ZnO/IDT/SiO2/金刚石SAW乳腺癌抗原免疫传感器研究

Breast cancer is the second high mortality disease next to angiocardiopathy. It is an important life science question which endangers women's health and life. CA15.3 is one of the important breast cancer biomarkers. Developing a label-free and high sensitive sensor for CA15.3 is a key to diagnosis breast cancer early and save life. .In this project, we will construct a Love wave SAW sensor using Au nanoparticles-graphene composite as sensitive film and high freqency ZnO/IDT/SiO2/diamond surface acoustic wave (SAW) delay line as a base device. The sensor will realize the fast and accurate detection of CA15.3 and improve the detection efficiency and precision to serum. The velocity, electromechanical coupling factor, temperature coefficient of frequency, and sensitivity versus the thicknesses of ZnO and SiO2 are given. The dependence of sensitivity, Au nanoparticles and graphene will be studied and the synergistic effect of Au nanoparticles and graphene to improve immune response will be discussed.

乳腺癌是仅次于心血管病的第二个高死亡率疾病，已成为严重影响妇女身心健康甚至危及生命的重大生命科学问题。CA15.3是最重要的乳腺癌肿瘤标志物之一，开发一种高灵敏的无标记CA15.3检测传感器，实现早期诊断，是挽救生命的关键。.本项目以“金纳米粒子-石墨烯”作为敏感膜，以高频“ZnO/IDT/SiO2/金刚石”SAW延迟线作为基础器件，构建无酶型Love波SAW生物传感器，实现对CA15.3的灵敏、快速测定，以提高对血清的检测效率和精度；研究 “ZnO/IDT/SiO2/金刚石”多层膜结构中Love波相速度、机电耦合系数、频率温度系数和传感灵敏度与ZnO膜厚度、SiO2膜厚度的关系；分析SAW传感器的灵敏度、金纳米粒子及石墨烯性能之间的依赖关系，研究Au纳米粒子和石墨烯共同促进免疫反应的协同作用机理。

乳腺癌是仅次于心血管病的第二个高死亡率疾病，已成为严重影响妇女身心健康甚至危及生命的重大生命科学问题。CA15.3是最重要的乳腺癌肿瘤标志物之一，开发一种高灵敏的无标记CA15.3检测传感器，实现早期诊断，是挽救生命的关键。.本项目以“金纳米粒子-石墨烯”作为敏感膜，以高频“ZnO/IDT/SiO2/金刚石”SAW延迟线作为基础器件，构建无酶型SAW生物传感器，实现对CA15.3的测定。首先，采用有限元法研究了 “ZnO/IDT/SiO2/金刚石”多层膜结构中Rayleigh和Love波SAW的相速度、机电耦合系数和频率温度系数与ZnO膜取向、ZnO膜厚度、SiO2膜厚度的关系，给出了优化的结构参数，在此基础上，模拟了延迟线结构的SAW器件；其次，摸索了“ZnO/IDT/SiO2/金刚石”多层膜结构SAW器件的制备工艺和“金纳米粒子-石墨烯”敏感膜的制备工艺，给出了优化的工艺参数。最后，实现了对CA15.3的测定，分析了Au纳米粒子和石墨烯共同促进免疫反应的协同作用机理。