耐盐好氧颗粒污泥EPS絮凝活性与丝状菌生长的响应关系

Against the problems of the loss of stability of haloduric aerobic granules on account of filament overgrowth. From the point of view of the flocculability of extracellular polymeric substances from haloduric aerobic granular sludge. The mycelia pellets, the hyphomycetic granular sludge and the haloduric aerobic granular sludge were chosen as the study object, the research was performed on characterisation of microbial granule from the formation into the process of disintegration, cleared the composition of flocculating activity of extracellular polymeric substances. and analyzed the effect of flocculability of extracellular polymeric substances on formation and stabilization of haloduric aerobic granular sludge. As to the relationship between the flocculating activity of extracellular polymeric substances and filament grow, shear force,organic loading and C/Nratio were investigated. clear the initiated factor of the filamentous fungi growing .The hyphomycetic granular sludge and the haloduric aerobic granular sludge with varied grain sizes were chosen as the study object, the results of treating wastewater and stability of microorganism particle with the excess of extracellular polymeric substances were investigated in the new sequencing batch reactor used hydraulic cyclone flow condition. The effect of process parameters on the flocculating activity of extracellular polymeric substances and the stability of haloduric aerobic granules was studied.clear the spatial distribution of flocculating activity of extracellular polymeric substances. and suited grain size of aerobic granule sludge was confirmed .the combined process of SBR and NSBR was used to treat with wastewater for removing organics efficiently. the research was performed on treating gibberellin wastewater , which received process parameters. which has some fundamental research significance in treatment of hyperhaline organic wastewater .

针对因丝状菌的过度生长而造成耐盐好氧颗粒污泥解体问题。从胞外多聚物〔EPS)絮凝性角度出发，研究菌丝球、丝状颗粒污泥和耐盐好氧颗粒污泥从形成到解体的过程，明晰EPS絮凝活性的主要成分，以期揭示EPS絮凝活性成分对耐盐好氧颗粒污泥形成及结构稳定的作用；研究不同工况下EPS中絮凝活性成分与丝状菌生长的关系，解析耐盐好氧颗粒污泥中丝状菌由密实生长向发散生长的引发因素；在带旋流装置的序批式反应器（NSBR）中，以不同粒径丝状颗粒污泥和好氧颗粒污泥为研究对象，研究上述微生物颗粒处理高盐废水效果和过量EPS对微生物颗粒稳定性影响，探讨工艺参数对EPS絮凝活性与微生物颗粒稳定的影响，明晰EPS絮凝活性的空间分布变化，并确定适宜高盐废水处理的好氧颗粒污泥粒径；在SBR和NSBR组成的废水处理系统中，探索好氧颗粒污泥稳定处理赤霉素废水的工艺参数。通过上述研究，将进一步丰富好氧颗粒污泥处理高盐废水理论。

针对因丝状菌的过度生长而造成耐盐好氧颗粒污泥解体问题。从胞外多聚物〔EPS)絮凝性角度出发，研究菌丝球、丝状颗粒污泥和耐盐好氧颗粒污泥从形成到解体的过程，探索好氧颗粒污泥稳定处理赤霉素废水的工艺参数。开展了以下研究工作。（1）以菌丝球、丝状菌形成的丝状菌颗粒污泥和添加微生物絮凝剂制备的好氧颗粒污泥为研究对象，研究上述微生物颗粒的形成过程，发现实验室分离的丝状菌最佳碳源是蔗糖，最佳氮源是氯化铵；对生长过程的EPS研究发现，EPS的成分与模拟废水氮源有一定的相关性，利用无机氮源作为培养成分，在前期主要成分是多糖，在后期，利用主要成分是多糖和蛋白质，而利用有机氮源作为培养成分，无论前期还是后期，EPS的主要成分都是多糖与蛋白质。利用扫描电镜观察到，颗粒污泥包裹着大量的EPS。（2）以培养获得的成熟菌丝球、丝状菌颗粒污泥和好氧颗粒污泥为研究对象，考察不同水力剪切力、溶解氧、有机负荷、盐度、C/N 条件下，不同粒径微生物颗粒出现解体的过程，研究表明有机负荷、溶解氧是丝状菌颗粒污泥密实成长转为发散成长的主要因素；适宜高盐废水处理的丝状颗粒污泥粒径为6mm，；实验室制备的好氧颗粒污泥为4mm。（3）在SBR 和带旋流装置的新型序批式反应器（NSBR）组成的高盐废水处理系统中，以生产赤霉素的高盐有机废水为基质，研究适宜粒径好氧颗粒污泥高效处理高盐有机废水效果，发现好氧颗粒污泥无法直接处理生产赤霉素的高盐有机废水，需调整废水水质，使C/N为10:1、盐含量为3%， pH为6～7，调整有机负荷率为1 KgCOD/(m3∙d)-5 KgCOD/(m3∙d)、温度为15℃-28℃之间，对废水COD去除率达到90%以上，但对总氮和总磷出除率较低。（4）本项目观察到颗粒污泥的另一种生长方式，大的颗粒污泥像桔子一样分瓣的，并在后期分生出大量的小椭圆形的颗粒污泥，其产生的机理有待进一步研究。