SmyD1在鳜鱼肌卫星细胞早期分化过程中的调控机理研究

As one of the most commercially important fish in China as well in some other Asia countries, the mandarin fish exhibits a quite lot of advantages, such as good meat texture, full scale of amino acid profile, especially high content of essential and flavor amino acids, high amount of unsaturated fatty acid composition, as well to its broad tolerances adapted to different aquaculture environment. Muscle satellite cells provide with new cellular resources for muscle growth and self-renewal. In this proposal, we used the Chinese mandarin fish as model system to reveal the molecular mechaisms of muscle satellite cell activation, proliferation and self-renewal during muscle development and gowth, based on SmyD1 function analysis. The project will be specifically focused on the following aims: 1)to analyze morphological characterization of muscle satellite cells using histological techniques; 2) to investigate the SmyD1's expression profiles quantitively and qualititively, so as to reveal the relationship of SmyD1 expression and muscle satellite cell activation; 3)to study the histone methyltransferase SmyD1 and transcription factors Pax3, Pax7, Myf5 and MyoD regulatory networks in controlling muscle satellite cell proliferation and self-renewal. Collectively,these studies will make significant contribution to the current understanding of muscle satellite cell activation and proliferation in Chinese mandarin fish. More importantly, these findings also provide the technical implications for aquacultural application.

鳜鱼因其肉质细嫩，蛋白质含量高，已成为水产养殖业中重要养殖品种。肌卫星细胞是肌肉生长和修复过程中新肌纤维的来源。本研究聚焦甲基化酶SmyD1，在分离和鉴定鳜鱼肌卫星细胞基础上，通过实时定量PCR和Western-blotting分析SmyD1的表达变化规律及其与肌卫星细胞激活分化的内在联系，采用染色质免疫沉淀法和基因敲除方法阐明SmyD1在肌卫星细胞早期分化过程中对Pax3、Pax7、Myf5和MyoD的调控机制。该研究分别从分子、细胞和整体水平，运用生化和分子生物学等研究手段，分析鳜肌卫星细胞分化特征，揭示SmyD1在肌卫星细胞早期分化过程的调控机理，其研究结果不仅有助于我们更深一步了解鱼类肌肉胚后生长发育调控机制，而且将为我国鱼类高效健康养殖提供重要理论依据。

鳜鱼因其肉质细嫩，蛋白质含量高，已成为水产养殖业中重要养殖品种。肌卫星细胞是肌肉生长和修复过程中新肌纤维的来源。本研究克隆了甲基化酶SmyD1a和SmyD1b基因，通过实时定量PCR分析了他们的表达变化规律，分离和鉴定了鳜鱼肌卫星细胞，分析了鳜肌卫星细胞分化特征，其研究结果不仅有助于我们更深一步了解鱼类肌肉胚后生长发育调控机制，而且将为我国鱼类高效健康养殖提供重要理论依据。